Abstract
Genes outside of the mouse major histocompatibility complex (H-2) were found to be capable of specifically reversing the previously described nonresponsiveness to hen egg-white lysozyme (HEL) owing to H-2 b immune response (Ir) genes. C3H.SW, BALB.B, and C57L, all of the H-2 b haplotype, showed responsiveness to HEL, but not to human lysozyme (H UL). Mapping of the reversing gene(s) was attempted by testing H-2 b recombinant inbred (RI) strains of mice carrying C3H, BALB, and C57L non-H-2 b genes. Analysis of the strain distribution pattern of responsiveness with both CXB and BXH RI strains was consistent with the location of the responsible site within the H-3 region on chromosome 2. The anti-HEL proliferative responsiveness in two H-3 congenic strains of mice, B10.C(28NX)SN and B10.C-H-3 cH-3 a, that have BALB/c genes within the H-3 region confirmed the mapping, as well as localized the reversing gene(s) near the Ir-2 gene. The data are discussed with regard to the site of expression of the reversing gene(s) and its mechanism of action.
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Abbreviations
- MHC:
-
major histocompatibility complex
- HEL:
-
hen egg-white lysozyme
- Ir :
-
immune response gene
- HUL:
-
human lysozyme
- SDP:
-
strain distribution pattern
- PFC:
-
plaque-forming cells; β 2 m, β 2-microglobulin
- CFA:
-
complete Freund's adjuvant
- PT-LN:
-
parathymic lymph nodes
- RI:
-
recombinant inbred mice
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Sadegh-Nasseri, S., Kipp, D.E., Taylor, B.A. et al. Selective reversal of H-2 linked genetic unresponsiveness to lysozymes. Immunogenetics 20, 535–546 (1984). https://doi.org/10.1007/BF00364356
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DOI: https://doi.org/10.1007/BF00364356