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Selective reversal of H-2 linked genetic unresponsiveness to lysozymes

I. Non-H-2 gene(s) closely linked to the Ir-2 locus on chromosome 2 permit(s) an antilysozyme response in H-2 b mice

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Abstract

Genes outside of the mouse major histocompatibility complex (H-2) were found to be capable of specifically reversing the previously described nonresponsiveness to hen egg-white lysozyme (HEL) owing to H-2 b immune response (Ir) genes. C3H.SW, BALB.B, and C57L, all of the H-2 b haplotype, showed responsiveness to HEL, but not to human lysozyme (H UL). Mapping of the reversing gene(s) was attempted by testing H-2 b recombinant inbred (RI) strains of mice carrying C3H, BALB, and C57L non-H-2 b genes. Analysis of the strain distribution pattern of responsiveness with both CXB and BXH RI strains was consistent with the location of the responsible site within the H-3 region on chromosome 2. The anti-HEL proliferative responsiveness in two H-3 congenic strains of mice, B10.C(28NX)SN and B10.C-H-3 cH-3 a, that have BALB/c genes within the H-3 region confirmed the mapping, as well as localized the reversing gene(s) near the Ir-2 gene. The data are discussed with regard to the site of expression of the reversing gene(s) and its mechanism of action.

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Abbreviations

MHC:

major histocompatibility complex

HEL:

hen egg-white lysozyme

Ir :

immune response gene

HUL:

human lysozyme

SDP:

strain distribution pattern

PFC:

plaque-forming cells; β 2 m, β 2-microglobulin

CFA:

complete Freund's adjuvant

PT-LN:

parathymic lymph nodes

RI:

recombinant inbred mice

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Sadegh-Nasseri, S., Kipp, D.E., Taylor, B.A. et al. Selective reversal of H-2 linked genetic unresponsiveness to lysozymes. Immunogenetics 20, 535–546 (1984). https://doi.org/10.1007/BF00364356

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  • DOI: https://doi.org/10.1007/BF00364356

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