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Influence of the H-2 u haplotype on immune function in F1 hybrid mice

II. F1 antiparent mixed lymphocyte reactivity

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Abstract

Recently we reported that antigen-primed T cells from (H-2 u × H-2 s)F1 and (H-2 u × H-2 q)F1 mice responded poorly in vitro to antigen in the context of antigen-presenting cells of the non-H-2 u parent. It was suggested that this effect might be due to unbalanced expression of parental antigens in the F1 hybrid with the result that the non-H-2u A antigens were greatly reduced or absent in these mice. If this were the case, non-H-2u Ia-A cells might be expected to stimulate a mixed lymphocyte reaction (MLR) when cultured with Fl responder cells. When tested, (SJL × PL)F1 responder cells reacted strongly to SJL stimulator cells. There was no significant reaction to PL stimulator cells. The use of major histocompatibility complex (MHC) congenic mice showed the stimulatory antigens to be associated with the MHC. The MLR could be blocked significantly by monoclonal A-specific antibody of the appropriate specificity. When a monoclonal antibody reactivewith a private epitope associated with As was used to probe for the presence of As on the surface of (SJL × PL)F1 spleen cells, no antigen could be detected, indicating loss or alteration of this antigen. These findings suggest that an alteration of the expression of the parental As molecule may be responsible for this phenomenon.

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Abbreviations

APC:

antigen-presenting cells

BSS:

balanced salt solution

CTL:

cytotoxic T lymphocyte

IL-2:

interleukin-2

MHC:

major histocompatibility complex

MLR:

mixed lymphocyte reaction

T2 :

suppressor T lymphocyte

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Authors and Affiliations

  1. Department of Microbiology/Immunology, Emory University School of Medicine, 30322, Atlanta, GA, USA

    Robert B. Fritz & Marianne J. Skeen

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  1. Robert B. Fritz
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  2. Marianne J. Skeen
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Fritz, R.B., Skeen, M.J. Influence of the H-2 u haplotype on immune function in F1 hybrid mice. Immunogenetics 25, 161–166 (1987). https://doi.org/10.1007/BF00344029

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  • DOI: https://doi.org/10.1007/BF00344029

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