Summary
We have found a null Adh1 allele which arose as a somaclonal variant following tissue culture of maize embryos carrying Adh1-1S and Adh1-1F alleles. Cloning and sequencing shows that the mutant allele derives from Adh1-1S and that there has been a single base change in the coding region of the gene which converts and AAG lysine codon to a TAG stop codon. The rate of nucleotide substitution (two per 218 embryos cultured) is much greater than normal mutation rates.
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References
Brettell RIS, Dennis ES, Scowcroft WR, Peacock WJ (1986) Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase. Mol Gen Genet 202:235–239
D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. Crit Rev Plant Sci 3:73–112
Davies PA, Pallotta MA, Ryan SA, Scowcroft WR, Larkin PJ (1986) Somaclonal variation in wheat: genetic and cytogenetic characterization of alcohol dehydrogenase 1 mutants. Theor Appl Genet 72:644–653
Dennis ES, Gerlach WL, Pryor AJ, Bennetzen JL, Inglis A, Llewellyn D, Sachs MM, Ferl RJ, Peacock WJ (1984) Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res 12:3983–4000
Freeling M (1976) Intragenic recombination in maize: pollen analysis methods and the effect of parental Adh1 + isoalleles. Genetics 83:701–717
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Larkin PJ (1986) Case histories of genetic variability in vitro: wheat and triticale. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol. 3, Plant Regeneration and Genetic Variability. Academic Press, New York, pp 367–383
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
McCoy TJ, Phillips RL, Rines HW (1982) Cytogenetic analysis of plants regenerated from oat (Avena sativa) tissue cultures; high frequency of partial chromosome loss. Can J Genet Cytol 24:37–50
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Peschke VM, Phillips RL, Gengenbach BG (1986) Transposable element activity in progeny of regenerated maize plants. In: Somers DA (ed) Proc VI Intern Cong Plant Tissue and Cell Culture, IAPTC, Minneapolis, p 285
Sachs MM, Dennis ES, Gerlach WL, Peacock WJ (1986) Two alleles of maize Alcohol dehydrogenase 1 have 3′ structural and poly(A) addition polymorphisms. Genetics 113:449–467
Sears RG, Deckard EL (1982) Tissue culture variability in wheat; callus induction and plant regeneration. Crop Sci 22:546–550
Schwartz D, Endo J (1966) Alcohol dehydrogenase polymorphism in maize-simple and compound loci. Genetics 53:709–715
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Communicated by H. Saedler
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Dennis, E.S., Brettell, R.I.S. & Peacock, W.J. A tissue culture induced Adh1 null mutant of maize results from a single base change. Mol Gen Genet 210, 181–183 (1987). https://doi.org/10.1007/BF00337777
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DOI: https://doi.org/10.1007/BF00337777