Abstract
A major goal in attempting to understand plant succession is to assess the implications of fungal and bacterial biomass changes over time as plant-soil systems develop. In this study, the soil fungal and bacterial biomass of three successional semi-arid steppe communities, sampled 4, 12, and 38 years after cultivation ended, were compared with an uncultivated native plant community using microscopic procedures. In the course of the succession, significant increases in fungal hyphal lengths occurred, reaching a maximum in the oldest successional (38-year) community. Active (cytoplasm filled) hyphae decreased along the chronosequence, with the native plant community having the lowest values. Similar decreases in active bacterial biomass values occurred. In contranst, microscopically determined total bacterial numbers did not differ in soils associated with the 4-year-old and native plant communities. The ratio of active bacterial to fungal biomass, which increased over the chronosequence tested in this study, appears to provide a valuable integrative measure of plant-soil resource system development and ecosystem maturity.
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References
Allen MF (1993) Microbial and phosphate dynamics in a restored shrub steppe in southwestern Wyoming. Rest Ecol 1:196–205
Anderson JPE, Domsch KH (1975) Measurement of bacterial and fungal contributions to respiration of selected agricultural and forest soils. Can J Microbiol 21:314–322
Anderson JPE, Domsch KH (1978) A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biol Biochem 10:215–221
Arnolds E (1991) Decline of ectomycorrhizal fungi in Europe. Agric Ecosyst Env 35:209–244
Babiuk LA, Paul EA (1970) The use of fluorescein isothionate in the determination of the bacterial biomass of grassland soil. Can J Microbiol 16:57–62
Beare MH, Pohlad BR, Wright DH, Coleman DC (1993) Residue placement and fungicide effects on fungal communities in conventional and no-tillage soils. Soil Sci Soc Am J 57:392–399
Brenneman J (1989) Long-term ecological research in the United States: a network of research sites, 5th edn. College of Forestry Resources AR-10, University of Washington, Seattle, Wash
Carroll GC, Wicklow DT (1992) The fungal community: Its organization and role in the ecosystem, 2nd edn. Marcel Dekker, New York
Chanway CP, Turkington R, Holl FB (1991) Ecological implications of specificity between plants and rhizosphere micro-organisms. Adv Ecol Res 21:121–169
Clarholm M (1985) Possible roles for roots, bacteria, protozoa and fungi in supplying nitrogen to plants. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil. Br Ecol Soc, Blackwell, Oxford, pp 107–121
Clark FE, Paul EA (1970) The microflora of grassland. Adv Agron 22:375–435
Duah-Yentumi S, Johnson DB (1986) Changes in soil microflora in response to repeated applications of some pesticides. Soil Biol Biochem 18:629–635
Gupta WSR, Germida JJ (1988) Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation. Soil Biol Biochem 20:777–786
Hall SJ, Raffaelli DG (1993) Food webs: Theory and reality. Adv Ecol Res 24:187–239
Harrison KG, Broecker WS, Bonani G (1993) The effect of changing land use on soil radiocarbon. Science 262:725–726
Hassink J, Bouwman LA, Zwart KB, Brussard L (1993) Relationships between habitable pore space, soil biota and mineralization rates in grassland soils. Soil Biol Biochem 25:47–55
Holland EA, Coleman DC (1987) Litter placement effects on microbial and organic matter dynamics in an agroecosystem. Ecology 68:425–433
Hunt HW, Coleman DC, Ingham ER, Ingham RE, Elliott ET, Moore JC, Rose SL, Reid CPP, Morley CR (1987) The detrital food web in a shortgrass prairie. Biol Fertil Soils 3:57–68
Ingham ER, Klein DA (1984a) Soil fungi: Measurement of hyphal length. Soil Biol Biochem 16:279–280
Ingham ER, Klein DA (1984b) Soil fungi: Relationships between hyphal activity and staining with fluorescein diacetate. Soil Biol Biochem 16:273–278
Insam H, Domsch KH (1988) Relationship between soil organic carbon and microbial biomass on chronosequences of reclamation sites. Microb Ecol 15:177–188
Klein DA (1992) The Rhizosphere. In: Lederberg J (ed) Encyclopedia of microbiology, vol 3. Academic Press, New York, pp 565–572
Klein DA, Frederick BA, Redente EF (1989) Fertilizer effects on microbial communities and organic matter in the rhizosphere of Sitanion hystrix and Agropyron smithii. Arid Soil Res Rehab 3:397–404
Lockwood JL, Filonow AB (1981) Responses of fungi to nutrient-limiting conditions and to inhibitory substances in natural habitats. Adv Microb Ecol 5:1–153
Lodge DJ, Ingham ER (1991) A comparison of agar film techniques for estimating fungal biovolumes in litter and soil. Agric Ecosyst Environ 34:131–144
McLendon T, Redente EF (1992) Effects of nitrogen limitation on species replacement dynamics during early secondary succession on a semiarid sagebrush site. Oecologia 91:312–317
Nakas JP, Klein DA (1980) Mineralization capacity of bacteria and fungi from the rhizosphere-rhizoplane of a semiarid grassland. Appl Environ Microbiol 39:113–117
Nordgren A, Bååth E, Söderström B (1983) Microfungi and microbial activity along a heavy metal gradient. Appl Environ Microbiol 45:1829–1837
Pastor J, Gardner RH, Dale VH, Post WM (1987) Successional changes in nitrogen availability as a potential factor contributing to spruce declines in boreal North America. Can J For Res 17:1394–1400
Paustian K, Schnürer J (1987) Fungal growth response to carbon and nitrogen limitation: A theoretical model. Soil Biol Biochem 19:613–620
SAS Institute (1985) SAS user's guide: Statistics version 5. Cary, NC
Smith JL, Paul EA (1990) The significance of soil microbial biomass estimations. Soil Biochem 6:357–396
Stamatiadis S, Doran JW, Ingham ER (1990) Use of staining and inhibitors to separate fungal and bacterial activity in soil. Soil Biol Biochem 22:81–88
Swift MJ (1976) Species diversity and the structure of microbial communities in terrestrial habitats. In: Anderson JM, Macfadyen A (eds) The role of terrestrial and aquatic organisms in decomposition processes. Blackwell, Oxford, pp 185–222
Vance ED, Brookes PC, Jenkinson DS (1987) An extractive method for measuring soil microbial C. Soil Biol Biochem 19:703–707
Van Veen JA, Paul EA (1979) Conversion of biovolume measurements of soil organisms, grown under various moisture tensions, to biomass and their nutrient content. Appl Environ Microbiol 37:686–692
Warcup JH (1967) Fungi in soil. In: Burges A, Raw F (eds) Soil biology. Academic Press, New York, pp 51–110
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Klein, D.A., McLendon, T., Paschke, M.W. et al. Saprophytic fungal-bacterial biomass variations in successional communities of a semi-arid steppe ecosystem. Biol Fertil Soils 19, 253–256 (1995). https://doi.org/10.1007/BF00336168
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DOI: https://doi.org/10.1007/BF00336168