Summary
We have defined a new gene, alt, which affects RNA synthesis in Escherichia coli. Mutants for alt arise among revertants of strains lacking the CRP-cAMP system necessary for full expression of catabolite-sensitive operons. Studies on a temperature-sensitive alt mutant indicate that the alt gene product is necessary for the synthesis of an important class of messenger RNA molecules.
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Arditti, R. R., Grodzicker, T., Beckwith, J. R.: Evidence for two sites in the lac + promoter region. J. Mol. Biol. In press.
Berman, M., Lin, E. C. C.: Glycerol specific revertants of a phosphoenol-pyruvate phosphotransferase mutant: suppression by the desensitization of glycerol kinase to feedback inhibition. J. Bact. 105, 113–120 (1971).
Bremer, H., Yuan, D.: Uridine transport and incorporation into nucleic acids of Escherichia coli. Biochim. biophys. Acta (Amst.) 169, 21–34 (1968).
Cashel, M., Gallant, J.: Two compounds implicated in the function of the RC gene of Escherichia coli. Nature (Lond.) 221, 830–841 (1969).
Crombrugghe, B. de, Chen, B., Anderson, W., Nissley, P., Gottesman, M., Pastan, I., Perlman, R.: Lac DNA, RNA polymerase and cyclic AMP receptor protein cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription. Nature (Lond.) New Biology 231, 139–142 (1971).
Crombrugghe, B. de, Perlman, R. L., Varmus, H. E., Pastan, I.: Regulation of inducible enzyme synthesis in Escherichia coli by cyclic AMP. J. biol. Chem. 244, 5828–5835 (1969).
Emmer, M., Crombrugghe, B. de, Pastan, I., Perlman, R.: Cyclic AMP receptor protein of E. coli: Its role in the synthesis of inducible enzymes. Proc. nat. Acad. Sci. (Wash.) 66, 780–787 (1970).
Eron, L., Block, R.: Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 1828–1832 (1971).
Haan, P. G. de, Gross, J. D.: Transfer delay and chromosome withdrawal in conjugation in E. coli K12. Genet. Res. 3, 251–272 (1962).
Lederberg, E., Lederberg, J.: Genetic studies of lysogenicity in E. coli. Genetics 38, 51–64 (1953).
Low, B.: Formation of merodiploids in rec - recipient strains of E. coli K12. Proc. nat. Acad. Sci. (Wash.) 60, 160–167 (1968).
Magasanik, B.: Glucose effects, inducer exclusion and repression. In: The Lac Operon, eds. Beckwith and Zipser. Cold Spring Harbor 1970.
Masters, M., Broda, P. M. A.: Evidence for the bidirectional replication of the E. coli chromosome. Nature (Lond.) 232, 137–140 (1971).
Matney, T. S., Goldschmidt, E. P., Erwin, N. S.: The specificity of genomic sites affording registry with the fertility episomes in E. coli. Biochem. biophys. Res. Commun. 12, 483–486 (1963).
Pardee, A. B., Jacob, F., Monod, J.: The genetic control and cytoplasmic expression of “inducibility” on the synthesis of β-galactosidase by E. coli. J. molec. Biol. 1, 165–178 (1959).
Pato, M. L., Meyenberg, K. von: Residual RNA synthesis in Escherichia coli after inhibition of initiation of transcription by rifampicin. Cold Spr. Harb. Symp. quant. Biol. 35, 497–504 (1970).
Perlman, R., Chen, B., Crombrugghe, B. de, Emmer, M., Gottesman, M., Varmus, H., Pastan, I.: The regulation of lac operon transcription by cyclic adenosine 3′, 5′-monophosphate. cold Spr. Harb. Symp. quant. Biol. 35, 719–723 (1970).
Perlman, R. L., Crombrugghe, B. de, Pastan, I.: Cyclic AMP regulates transient and catabolite repression. Nature (Lond.) 223, 810–812 (1969).
Perlman, R. L., Pastan, I.: Regulation of β-galactosidase synthesis in E. coli by cyclic adenosine 3′, 5′-monophosphate. J. biol. Chem. 243, 5420–5427 (1968).
Prival, M. J., Magasanik, B.: Resistance repression of histidine and proline oxidase during nitrogen limited growth of Klebsiella aerogenes. J. biol. Chem. 246, 6288–6296 (1971).
Randerath, K.: Thin layer chromatography. New York: Academic Press 1966.
Revel, H., Luria, S., Rotman, B.: Biosynthesis of β-galactosidase controlled by phagecarried genes. I. Induced β-D-galactosidase biosynthesis after transduction of gene Z+ by a phage. Proc. nat. Acad. Sci. (Wash.) 47, 1956–1967 (1961).
Riggs, A. D., Reiness, G., Zubay, G.: Purification and DNA-binding properties of the catabolite activator protein. Proc. nat. Acad. Sci. (Wash.) 68, 1222–1225 (1971).
Scaife, J., Pekhov, A. P.: The deletion of chromosomal markers in association with F-prime formation in E. coli K12. Genet. Res. 5, 495–498 (1964).
Schwartz, D., Beckwith, J. R.: Mutants missing a factor necessary for the expression of catabolite sensitive operons in E. coli. In: The Lac Operon, eds. D. Zipser and J. R. Beckwith. Cold Spring Harbor 1970.
Silverstone, A. E., Arditti, R. R., Magasanik, B.: Catabolite insensitive revertants of lac promoter mutants. Proc. nat. Acad. Sci. (Wash.) 66, 773–779 (1970).
Silverstone, A. E., Magasanik, B., Reznikoff, W. S., Miller, J. H., Beckwith, J. R.: Catabolite sensitive sites of the lac operon. Nature (Lond.) 221, 1012–1014 (1969).
Taylor, A. L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155–175 (1970).
Travers, A. A., Kamen, R. I., Schleif, R. F.: Factor necessary for ribisomal RNA synthesis. Nature (Lond.) 228, 748–751 (1970).
Tyler, B., Magasanik, B.: Molecular basis of transient repression of β-galactosidase in Escherichia coli. J. Bact. 97, 550–556 (1969).
Ullman, A., Monod, J.: Cyclic AMP as an antagonist of catabolite repression in E. coli. FEBS Lett., 2, 57–60 (1968).
Yokota, T., Gots, J. S.: Requirement for cAMP for flagella formation in E. coli and S. typhimurium. J. Bact. 103, 513–516 (1970).
Zubay, G., Schwartz, D., Beckwith, J. R.: Mechanism of activation of catabolite-sensitive genes. A positive control system. Proc. nat. Acad. Sci. (Wash.) 66, 104–110.
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Silverstone, A.E., Goman, M. & Scaife, J.G. ALT: A new factor involved in the synthesis of RNA by Escherichia coli . Molec. gen. Genet. 118, 223–234 (1972). https://doi.org/10.1007/BF00333459
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DOI: https://doi.org/10.1007/BF00333459