Summary
Continuing the genetic and biochemical characterization of the streptomycin-resistant Escherichia coli mutant LD1, we confirmed that LD1 is temperature-sensitive for suppression of nonsense codons, and that this phenotype of the mutant and its streptomycin-resistance are genetically linked and are probably caused by a single mutation, strA (LD1). We also isolated a spontaneous revertant, called LD1-R, which partially relieves the restriction of nonsense suppression caused by the strA (LD1) mutation. LD1-R is derived by an additional mutation (revA) which is closely linked to strA (LD1). We further demonstrate that the weak suppression of a lacZUGA mutation in a suppressor-free strain, which probably takes place by normal tRNATrp, can be detected by the use of the chromagenic substance x-gal (5-Bromo-4-chloro-3-indolyl-β-d-Galactopyranoside).
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Engelberg-Kulka, H., Amiel, A., Dekel, L. et al. Genetic analysis of a streptomycin-resistant Escherichia coli mutant temperature-sensitive for nonsense suppression. Molec Gen Genet 188, 149–155 (1982). https://doi.org/10.1007/BF00333010
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DOI: https://doi.org/10.1007/BF00333010