Summary
In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition.
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Bolivar F, Rodriguez RL, Green PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system. Gene 2:95–106
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehi les derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156
Datta N, Hedges RW (1972) Trimethoprim resistance conferred by plasmids in Enterobacteriaceae. J Gen Microbiol 72:349–356
de la Cruz F, Grinsted J (1982) Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100. 1. J Bacteriol 151:222–228
Diaz-Aroca E, de la Cruz, F, Zabala JC, Ortiz JM (1984) Characterization of the new insertion sequence IS91 from an alpha-hemolytic plasmid of Escherichia coli. Mol Gen Genet 193:493–499
Diver WP, Grinsted J, Fritzinger DC, Brown NL, Altenbuchner J, Rogowsky P, Schmitt R (1983) DNA sequences of and complementation by the tmpR genes of Tn21, Tn501 and Tn1721. Mol Gen Genet 191:189–193
Galas DJ, Chandler M (1981) On the molecular mechanisms of transposition. Proc Nat Acad Sci USA 78:4858–4862
Grinsted J, de la Cruz F, Altenbuchner J, Schmitt R (1982) Complementation of transposition of tnpA mutants of Tn3, Tn21, Tn501 and Tn1721. Plasmid 8:276–286
Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E (1979) DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell 18:1153–1163
Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998
Kleckner N (1981) Transposable elements in procaryotes. Annu Rev Genet 15:341–404
Mötsch S, Schmitt R (1984) Replicon fusion mediated by a singleended derivative of transposon Tn1721. Mol Gen Genet 195:281–287
Novick RP, Clowes RC, Cohen SN, Curtiss R, Datta N, Falkow S (1976) Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev 40:168–189
Rogowsky P, Schmitt R (1984) Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the resolution site. Mol Gen Genet 193:162–166
Viera J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Willetts N, Johnson D (1981) pED100, a conjugative F plasmid derivative without insertion sequences. Mol Gen Genet 182:520–522
Zheng ZX, Chandler M, Hipskind R, Clerget M, Caro L (1981) Dissection of the r-determinant of R100.1: the sequence at the extremities of Tn21. Nucleic Acids Res 9:6265–6278
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Communicated by A. Böck
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Avila, P., de la Cruz, F., Ward, E. et al. Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase. Mol Gen Genet 195, 288–293 (1984). https://doi.org/10.1007/BF00332761
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DOI: https://doi.org/10.1007/BF00332761