Summary
Lambda phages carrying the R100 finO gene have been isolated from an R100::“ cointegrate in which λ was inserted into the R100 traD gene at kb coordinate 72.1. Physical analyses of these phages place the finO gene within R100 SalI fragment D, near kb coordinate 82.0. Analysis of proteins synthesized by the phages did not identify the finO gene product, although a constitutive protein of m.w. 30,100 was encoded by R100 DNA between kb coordinates 78.7 and 81.2.
Similar content being viewed by others
References
Achtman M, Willetts N, Clark A (1971) Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol 106:529–538
Dempsey WB (1969) Characterization of pyridixine auxotrophs of E. coli: P1 transduction. J Bacteriol 97:1403–1410
Dempsey WB, McIntire S, Willetts N, Schottel J, Kinscherf TG, Silver S, Shannon WA Jr (1978) Properties of lambda transducing bacteriophages carrying R100 plasmid DNA: Mercury resistance genes. J Bacteriol 136:1084–1093
Dempsey W, McIntire SA (1979) Lambda transducing phages derived from a FinO- R100::λ cointegrate plasmid: Proteins encoded by the R100 replication/incompatibility region and the antibiotic resistance determinant. Mol Gen Genet 76:319–334
Dempsey W, Willetts NS (1976) Plasmid cointegrates of prophage lambda and R factor R100. J Bacteriol 126:166–176
DeWilde M, Davies JE, Schmidt FJ (1978) Low-molecular-weight RNA species coded by plasmid NR1. In: Schlessinger D (ed) Microbiology 1978. Am Soc Microbiol, Washington DC, pp 188–191
Egawa R, Hirota Y (1962) Inhibition of fertility by multiple drug resistance factor in E. coli K12. Japan J Genet 37:66–69
Finnegan D, Willetts N (1972) The nature of the transfer inhibitor of several F-like plasmids. Mol Gen Genet 119:57–66
Finnegan D, Willetts N (1973) The site of action of the N transfer inhibitor. Mol Gen Genet 127:307–316
McIntire S, Willetts N (1978) Plasmid cointegrates of Flac and lambda prophage. J Bacteriol 134:184–192
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York
Rosen J, Ryder T, Inokuchi H, Ohtsubo H, Ohtsubo E (1980) Genes and sites involved in replication and incompatibility of an R100 plasmid derivative based on nucleotide sequence analysis. Mol Gen Genet 179:527–537
Shimada K, Weisberg RA, Gottesman ME (1972) Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol 63:483–503
Timmis KN, Andres I, Achtman M (1978) Fertility repression of F-like conjugative plasmids: Physical mapping of the R6-5 finO and finP cistrons and identification of the finO protein. Proc Natl Acad Sci USA 75:5836–5840
Watanabe T, Nishida H, Ogata C, Arai T, Sato S (1964) Episomemediated transfer of drug-resistance in Enterobacteriaceae. VII. Two types of naturally occuring R-factors. J Bacteriol 88:716–726
Willetts N (1977) The transcriptional control of fertility in F-like plasmids. J Mol Biol 112:141–148
Willetts N, McIntire SA (1978) Isolation and characterization of λtra transducing phages from EDFL223 (FlactraB::EDλ4). J Mol Biol 126:525–549
Willetts N, Skurray R (1980) The conjugation system of F-like plasmids. Ann Rev Genet 14:41–76
Author information
Authors and Affiliations
Additional information
Communicated by G.O'Donovan
Rights and permissions
About this article
Cite this article
Dempsey, W.B., McIntire, S.A. The finO gene of antibiotic resistance plasmid R100. Mol Gen Genet 190, 444–451 (1983). https://doi.org/10.1007/BF00331075
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331075