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An ordered arrangement of chromosomes in the somatic nucleus of common wheat, Triticum aestivum L.

I. Spatial relationships between chromosomes of the same genome

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Abstract

Spatial relationships between chromosomes of the same genome, both homologous and non-homologous, were studied in root-tip cells of common wheat, Triticum aestivum (2n = 6x = 42). Mean distance between members of all the 21 homologous pairs (seven in each of the three genomes) and of 45 out of the 63 possible non-homologous combinations of two (21 in each genome) were determined. To minimize disruption of nuclear chromosomal arrangement, the cells were pretreated with cold temperature either in tap water or in a physiological medium (White solution) and distances between cytologically marked chromosomes were measured at metaphase. Comparison of distances for homologues with those for non-homologues indicated clearly that, within each genome, the homologous chromosomes were significantly closer to one another than were the non-homologues. Distances between homologues were similar in all three genomes, as were distances between non-homologues. The data are consistent with the hypothesis that the chromosomes of each genome of common wheat are arranged in the somatic nucleus in a highly specific ordered pattern. In this hypothetical arrangement, homologous chromosomes are closely associated, while the nonhomologues occupy definite positions with respect to one another. The universality of the phenomenon and its cellular mechanism and biological significance are discussed.

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References

  • Ashburner, M.: Gene activity dependent on chromosome synapsis in the polytene chromosomes of Drosophila melanogaster. Nature (Lond.) 214, 1159–1160 (1967)

    Google Scholar 

  • Ashley, T., Wagenaar, E.B.: End to end attachment of haploid chromosomes of Ornithogalum virens. Canad. J. Genet. Cytol. 14, 716–717 (1972)

    Google Scholar 

  • Ashley, T., Wagenaar, E.B.: Telomeric associations of gametic and somatic chromosomes in diploid and autotetraploid Ornithogalum virens. Canad. J. Genet. Cytol. 16, 61–67 (1974)

    Google Scholar 

  • Avivi, L.: The effect of genes controlling different degrees of homoeologous pairing on quadrivalent frequency in induced autotetraploid lines of Triticum longissimum. Canad. J. Genet. Cytol. 18, 357–364 (1976a)

    Google Scholar 

  • Avivi, L.: Colchicine induced bivalent pairing of tetraploid microsporocytes in Triticum longissimum and T. speltoides. Canad. J. Genet. Cytol. 18, 731–738 (1976b)

    Google Scholar 

  • Avivi, L., Feldman, M.: The mechanism of somatic association in common wheat, Triticum aestivum L. IV. Further evidence for modification of spindle tubulin through the somatic association genes as measured by vinblastine binding. Genetics 73, 379–385 (1973a)

    Google Scholar 

  • Avivi, L., Feldman, M.: Mechanism of non-random chromosome placement in common wheat. Proc. 4th. Inter. Wheat Genet. Symp., pp. 627–633 (1973b)

  • Avivi, L., Feldman, M.: Arrangement of chromosomes in the interphase nucleus of plants. Hum. Genet. 55, 281–295 (1980)

    Google Scholar 

  • Avivi, L., Efron, Y., Feldman, M.: Effect of somatic chromosomal association on the zymogram of alcohol dehydrogenase (ADH) in common wheat. Genetics 71 (Suppl.), 2–3 (1972)

    Google Scholar 

  • Avivi, L., Feldman, M., Bushuk, W.: The mechanism of somatic association in common wheat, Triticum aestivum L. I. Suppression of somatic association by colchicine. Genetics 62, 745–752 (1969)

    Google Scholar 

  • Avivi, L., Feldman, M., Bushuk, W.: The mechanism of somatic association in common wheat, Triticum aestivum L. II. Differential sensitivity to colchicine of spindle microtubules of plants having different doses of the somatic association genes. Genetics 65, 585–592 (1970a)

    Google Scholar 

  • Avivi, L., Feldman, M., Bushuk, W.: The mechanism of somatic association in common wheat, Triticum aestivum L. III. Differential affinity for nucleotides of spindle microtubules of plants having different doses of the somatic association suppressor. Genetics 66, 449–461 (1970b)

    Google Scholar 

  • Avivi, L., Feldman, M., Brown, M.: An ordered arrangement of chromosomes in the somatic nucleus of common wheat, Triticum aestivum L. II. Spatial relationships between chromosomes of different genomes. Chromosoma (Berl.) 86, 17–26 (1982)

    Google Scholar 

  • Barton, D.E., David, F.N., Merrington, M.: The relative positions of the chromosomes in the human cell in mitosis. Ann. Hum. Genet. 29, 139–146 (1965)

    Google Scholar 

  • Bennett, D.: Non-random association of chromosomes during mitotic metaphase in tissue cells of the mouse. Cytologia (Tokyo) 31, 411–415 (1966)

    Google Scholar 

  • Chauhan, K.P.S., Abel, W.O.: Evidence for the association of homologous chromosomes during premeiotic stages in Impatiens and Salvia. Chromosoma (Berl.) 25, 297–302 (1968)

    Google Scholar 

  • Comings, D.E.: The rationale for an ordered arrangement of chromatin in the interphase nucleus. Amer. J. Hum. Genet. 20, 440–460 (1968)

    Google Scholar 

  • Comings, D.E.: Arrangement of chromatin in the nucleus. Hum. Genet. 53, 131–143 (1980)

    Google Scholar 

  • Darvey, N.L., Driscoll, C.J.: Evidence against somatic association in hexaploid wheat. Chromosoma (Berl.) 36, 140–149 (1972)

    Google Scholar 

  • Dubuc, J.P., McGinnis, R.C.: Somatic association in Avena sativa L. Science 167, 999–1000 (1970)

    Google Scholar 

  • Dvorak, J., Knott, D.R.: A study of somatic association of wheat chromosomes. Canad. J. Genet. Cytol. 15, 411–416 (1973)

    Google Scholar 

  • Evans, H.J., Bigger, T.R.L.: Chromatid aberrations induced by gamma irradiation. II. Non randomness in the distribution of chromatid aberrations in relation to chromosome length in Vicia faba root-tip cells. Genetics 46, 277–289 (1961)

    Google Scholar 

  • Fedak, G., Helgason, S.B.: Somatic association of chromosomes in barley. Canad. J. Genet. Cytol. 12, 496–500 (1970)

    Google Scholar 

  • Feldman, M.: The effect of chromosomes 5 B, 5 D and 5 A on chromosomal pairing in Triticum aestivum. Proc. Natl. Acad. Sci. (Wash.) 55, 1447–1453 (1966)

    Google Scholar 

  • Feldman, M.: Regulation of somatic association and meiotic pairing in common wheat. Proc. 3rd Inter. Wheat Genet. Symp. (Austral. Acad. Sci., Canberra), 169–178 (1968)

  • Feldman, M., Avivi, L.: Non-random arrangement of chromosomes in common wheat. In: Chromosomes today 4, pp. 187–196 (1973a)

    Google Scholar 

  • Feldman, M., Avivi, L.: The pattern of chromosomal arrangement in nuclei of common wheat and its genetic control. Proc. 4th Inter. Wheat Genet. Symp., Missouri Agr. Exp. Sta., Columbia, Mo., pp. 675–684 (1973b)

  • Feldman, M., Mello-Sampayo, T., Avivi, L.: Somatic association of homoeologous chromosomes in Triticum aestivum. Chromosoma (Berl.) 37, 209–222 (1972)

    Google Scholar 

  • Feldman, M., Mello-Sampayo, T., Sears, E.R.: Somatic association in Triticum aestivum. Proc. Natl. Acad. Sci. (Wash.) 56, 1192–1199 (1966)

    Google Scholar 

  • Galperin, H.: Etude de la distribution generale des 46 chromosomes dans les cellules humaines en metaphase. Humangenetik 6, 118–130 (1968).

    Google Scholar 

  • Gerassimova, H.: Fertilization in Crepis capillaris (L.) Wall. Cellule 42, 103–148 (1933)

    Google Scholar 

  • Godin, D.E., Stack, S.M.: Heterochromatic connectives between the chromosomes of Secale cereale. Canad. J. Genet. Cytol. 17, 269–273 (1975)

    Google Scholar 

  • Grell, R.F.: Meiotic and somatic pairing. In: Genetic Organization, A Comprehensive Treatise, Vol. 1, pp. 361–492 (E.W. Caspari and A.W. Ravin, eds.). New York: Academic Press 1969

    Google Scholar 

  • Heneen, W.K., Nichols, W.W.: Non-random arrangement of metaphase chromosomes in cultured cells of the Indian deer Muntiacus mutjak. Cytogenetics 11, 153–164 (1972)

    Google Scholar 

  • Horn, J.D.: Somatic association as a general phenomenon in maize. Maize Genet. Coop. Newsl. 44, 198–200 (1970)

    Google Scholar 

  • Horn, J.D.: Somatic association: the effects of various methods of arresting spindle-fiber development. Maize Genet. Coop. Newsl. 45, 209–210 (1971)

    Google Scholar 

  • Horn, J.D.: Somatic association and an organized nucleus in Zea mays L. Ph. D. Thesis, University of Western Ontario (1973)

  • Horn, J.D.: Primary non-homologue association among somatic chromosomes. Maize Genet. Coop. Newsl. 49, 155–156 (1975)

    Google Scholar 

  • Horn, J.D., Walden, D.B.: Affinity distance values among somatic metaphase chromosomes in maize. Genetics 88, 181–200 (1978)

    Google Scholar 

  • Huskins, C.L., Smith, S.G.: A cytological study of the genus Sorghum Pers. I. The somatic chromosomes. J. Genet. 25, 241–249 (1932)

    Google Scholar 

  • Janaki-Ammal, E.K.: Chromosome studies in Nicandra physaloides. La Cellule 41, 87–110 (1932)

    Google Scholar 

  • Jancey, R.C., Walden, D.B.: Analysis of pattern in distribution of breakage points in the chromosomes of Zea mays L. and D. melanogaster Meigen. Canad. J. Genet. Cytol. 14, 429–442 (1972)

    Google Scholar 

  • Jancey, R.C.: A new source of evidence for the polarized nucleus in maize. Canad. J. Genet. Cytol. 17, 245–252 (1975)

    Google Scholar 

  • Kaina, B., Rieger, R., Michaelis, A., Schubert, I.: Effects of chromosome repatterning in Vicia faba L. IV. Chromosome constitution and its bearing on the frequency and distribution of chromatid aberrations. Biol. Zbl. 98, 271–283 (1979)

    Google Scholar 

  • Kihara, H.: Artifically raised haploids and their uses in plant breeding. Sciken Ziho 27–28, 14–29 (1979)

    Google Scholar 

  • Kirsch-Volders, M., Hens, L., Susanne, C.: Telomere and centromere association tendencies in the human male metaphase complement. Hum. Genet. 54, 69–77 (1980)

    Google Scholar 

  • Kitani, Y.: Orientation, arrangement and association of somatic chromosomes. Jap. J. Genet. 38, 244–256 (1963)

    Google Scholar 

  • Kumar, S., Natarajan, A.T.: Kinetics of two-break chromosome exchanges and the spatial arrangement of chromosome strands in interphase nucleus. Nature (Lond.) 209, 796–797 (1966)

    Google Scholar 

  • Kushnir, U.: Behavior of alien chromosomal material in the wheat genome. Thesis for the degree of Doctor of Philosophy. The Weizmann Institute of Science, Rehovot (1977)

  • Kuwada, U.: A cytological study of Oryza sativa L. Bot. Mag. Tokyo 24, 267–281 (1910)

    Google Scholar 

  • Lewis, E.G.: The theory and application of a new method of detecting chromosomal rearrangements in Drosophila melanogaster. Amer. Naturalist 88, 225–239 (1954)

    Google Scholar 

  • Maguire, M.P.: Evidence for homologous pairing of chromosomes prior to meiotic prophase in maize. Chromosoma (Berl.) 21, 221–231 (1967)

    Google Scholar 

  • McLeish, J.: The action of maleic hydrazide in Vicia. Heredity (Suppl.) 6, 125–147 (1953)

    Google Scholar 

  • Mello-Sampayo, T.: Somatic association between telocentrics for both arms of the same chromosome in Triticum aestivum. Proc. XIIth Intern. Congr. Genet. 1, 163 (1968)

    Google Scholar 

  • Mello-Sampayo, T.: Somatic association of telocentric chromosomes carrying homologous centromeres in common wheat. Theor. Appl. Genet. 43, 174–181 (1973)

    Google Scholar 

  • Michaelis, A., Rieger, R.: On the distribution between chromosomes of chemically induced chromatid aberrations: studies with a new karyotype of Vicia faba. Mutation Res. 6, 81–92 (1968)

    Google Scholar 

  • Miles, J.: Somatic association of homologues induced by abnormal chromosome 10. Maize Genet. Coop. Newsl. 42, 77–79 (1968)

    Google Scholar 

  • Miller, O.J., Berg, W.R., Mukherjee, B.B., Gamble, A. Van N., Christakas, A.C.: Non-random distribution of chromosomes in metaphase figures from cultured human leukocytes. II. The peripherial location of chromosomes 13, 17–18 and 21. Cytogenetics 2, 152–167 (1963)

    Google Scholar 

  • Mosolov, A.N.: Evolutional conception of nucleus and chromosome structure. The Nucleus (Clacutta) 27, 51–64 (1974)

    Google Scholar 

  • Naylor, A.F., Warburton, D., Warburton, F.E.: Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase. II. Analysis of variance of intercentromeric distances. Hum. Genet. 18, 307–313 (1973)

    Google Scholar 

  • Ockey, C.H.: The position of chromosomes at metaphase in human fibroblasts and their DNA synthesis behavior. Chromosoma (Berl.) 27, 308–320 (1969)

    Google Scholar 

  • Overton, J.B.: The organization of the nuclei in the root tips of Podophyllum peltatum. Trans. Wisconsin Acad. Sci. Arts. Letters 20, 275–322 (1922)

    Google Scholar 

  • Raicu, P., Vladescu, B., Kirilova, M.: Distribution of chromosomes in metaphase plates of Mesocricetus newtoni. Genet. Res. (Camb.) 15, 1–6 (1970)

    Google Scholar 

  • Rao, R.N., Natarajan, A.T.: Somatic association in relation to chemically induced chromosome aberrations in Vicia faba. Genetics 57, 821–835 (1967)

    Google Scholar 

  • Revell, S.H.: Chromosome breakage by X-rays and radiomimetic substances in Vicia. Heredity (Suppl.) 6, 107–124 (1953)

    Google Scholar 

  • Rieger, R., Michaelis, A., Schubert, I., Meister, A.: Somatic interphase pairing of Vicia chromosomes as inferred from the hom/het ratio of induced chromatid interchanges. Mutation Res. 20, 295–298 (1973)

    Google Scholar 

  • Rodman, T.C., Flehinger, B.J., Rohlf, F.J.: Metaphase chromosome associations: colcemid distorts the pattern. Cytogenet. Cell Genet. 27, 98–110 (1980)

    Google Scholar 

  • Rodman, T.C., Flehinger, B.J., Squire, R.D.: Patterns of association in the human metaphase complement: ring analysis and estimation of associativity of specific chromosome regions. Hum. Genet. 41, 19–34 (1978)

    Google Scholar 

  • Sadasivaiah, R.S., Watkins, R., Rajhathy, T.: Somatic association of chromosomes in diploid and hexaploid Avena. Chromosoma (Berl.) 28, 468–481 (1969)

    Google Scholar 

  • Sax, K.: Analysis of x-ray induced chromosomal aberrations in Tradescantia. Genetics 25, 41–68 (1940)

    Google Scholar 

  • Schulz-Schaeffer, J., Haun, C.R.: The chromosomes of hexaploid common wheat, Triticum aestivum L. Z. Pflanzenzucht. 46, 112–124 (1961)

    Google Scholar 

  • Shchapova, A.I.: On the karyotype pattern and the chromosome arrangement in the interphase nucleus. Tsitologia (USSR) 13, 1157–1164 (1971)

    Google Scholar 

  • Singh, D., Joshi, B.C.: Orientation of homoeologous chromosomes in the somatic cells of wheat. Wheat Inf. Serv. 33–34, 9 (1972)

    Google Scholar 

  • Singh, R.J., Röbbelen, G., Okamoto, M.: Somatic association at interphase studied by Giemsa banding technique. Chromosoma (Berl.) 56, 265–273 (1976)

    Google Scholar 

  • Stack, S.M., Brown, W.V.: Somatic and premeiotic pairing of homologues in Plantago ovata. Bull. Torrey Bot. Club 96, 143–149 (1969)

    Google Scholar 

  • Stack, S., Clarke, C.: Differential Giemsa staining of the telomeres of Allium cepa chromosomes: observations related to chromosome pairing. Canad. J. Genet. Cytol. 15, 619–624 (1973)

    Google Scholar 

  • Strasburger, E.: Die Apogamie der Eualchemillen. Jb. wiss. Bot. 41, 88–164 (1904)

    Google Scholar 

  • Strasburger, E.: Typische und allotypische Kernteilung. Histologische Beitrage zur Vererbungsfrage. Jb. wiss. J. Bot. 42, 1–71 (1905)

    Google Scholar 

  • Strasburger, E.: Über die Individualität der Chromosomen und die Pfropfhybriden-Frage. Jb. wiss. Bot. 44, 482–555 (1907)

    Google Scholar 

  • Strasburger, E.: Chromosomenzahl. Flora 100, 398–446 (1910)

    Google Scholar 

  • Sved, J.A.: Hybrid dysgenesis in Drosophila melanogaster: a possible explanation in terms of spatial organization of chromosomes. Aust. J. Biol. Sci. 29, 375–388 (1976)

    Google Scholar 

  • Thomas, H.: Somatic association of chromosomes in asynaptic genotypes of Avena sativa L. Chromosoma (Berl.) 42, 87–94 (1973)

    Google Scholar 

  • Vogel, F., Schroeder, T.M.: The internal order of the interphase nucleus. Humangenetik 25, 265–297 (1974)

    Google Scholar 

  • Wagenaar, E.B.: End-to-end chromosome attachments in mitotic interphase and their possible significance to meiotic chromosome pairing. Chromosoma (Berl.) 26, 410–426 (1969)

    Google Scholar 

  • Walden, D.B., Jancey, R.C.: Reassociation patterns among segmental interchanges in maize. Heredity 36, 293–304 (1976)

    Google Scholar 

  • Warburton, D., Naylor, A.F., Warburton, F.E.: Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase. I. Mean mterchromosomal distances and distances from cell center. Hum. Genet. 18, 297–306 (1973)

    Google Scholar 

  • Watkins, G.M.: A study of chromosome pairing in Yucca rupicola. Bull. Torrey Bot. Club 62, 133–150 (1935)

    Google Scholar 

  • Werry, P.A.Th.J., Stoffeisen, K., Engels, F.M., Van der Laan, F., Spanjers, A.W.: The relative arrangement of chromosomes in mitotic interphase and metaphase in Haplopappus gracilis. Chromosoma (Berl.) 62, 93–101 (1977)

    Google Scholar 

  • White, P.R.: The cultivation of animal and plant cells. (2nd ed.), p. 74. New York: Ronald Press Co., 1963

    Google Scholar 

  • Yoshida, H., Yamaguchi, M.: Arrangement and association of somatic chromosomes induced by chloramphenicol in barley. Chromosoma (Berl.) 43, 399–407 (1973)

    Google Scholar 

  • Yoshida, H., Yamaguchi, H., Takano, Y.: Somatic association of homologous chromosomes in rye. Jap. J. Genetics 49, 45–47 (1974)

    Google Scholar 

  • Yoshida, H., Yamamoto, K., Yamaguchi, H.: Fragmentation and nondisjunction of barley chromosomes after the treatment of chloramphenicol and cycloheximide. Cytologia (Tokyo) 37, 697–707 (1972)

    Google Scholar 

  • Zang, K.D., Back, E.: Quantitative studies on the arrangement of human metaphase chromosomes. I. Individual features in the association pattern of the acrocentric chromosomes of normal males and females. Cytogenetics 7, 455–470, (1968)

    Google Scholar 

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Authors and Affiliations

  1. Human Genetics, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Israel

    Lydia Avivi

  2. Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel

    Moshe Feldman

  3. Statistics Department, Tel-Aviv University, Ramat-Aviv, Israel

    Morton Brown

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  1. Lydia Avivi
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  2. Moshe Feldman
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  3. Morton Brown
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Avivi, L., Feldman, M. & Brown, M. An ordered arrangement of chromosomes in the somatic nucleus of common wheat, Triticum aestivum L.. Chromosoma 86, 1–16 (1982). https://doi.org/10.1007/BF00330726

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