Summary
The expression pattern of Sh and Sus, which encode sucrose synthase isozymes SS1 and SS2, respectively, and Adh-1, which encodes alcohol dehydrogenase 1 (ADH1), was examined in situ in the lower region of the primary root of maize seedlings in response to anaerobiosis. Anaerobic induction of ADH1 RNA was localized to the epidermis and cortex. Induction of Sh was marked by highly elevated SS1 RNA levels in the vascular elements, pith, and epidermis. A significant but less drastic increase in SS protein was found in these same tissues as well as the root cap; the increased level of immunosignal was restricted to cells within approximately 1 cm of the root apex. Sus responded to anaerobic stress with a slight reduction in SS2 RNA and protein levels except in the root cap where SS2 protein, but not SS2 RNA, was induced. Thus, in situ hybridization and/or immunolocalization experiments revealed a unique spatial pattern of expression for each of the three genes in response to anaerobiosis. Furthermore, we conclude that multiple regulatory controls including cell specific post-transcriptional mechanisms modulate SS levels in anaerobically stressed seedlings.
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Rowland, L.J., Chen, YC. & Chourey, P.S. Anaerobic treatment alters the cell specific expression of Adh-1, Sh, and Sus genes in roots of maize seedlings. Mol Gen Genet 218, 33–40 (1989). https://doi.org/10.1007/BF00330562
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DOI: https://doi.org/10.1007/BF00330562