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Identification of the minimal essential region for the replication origin of miniF plasmid

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Summary

The minimal replication origin of miniF plasmid was found to lie within a region of 217 bp in length. This region contains an AT cluster and the four 19 bp direct repeats responsible for incompatibility, termed incB. Its location coincides with that of ori2 of plasmid F, previously inferred to be the replication starting point by electron microscopic analysis (Eichenlaub et al. 1981).

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Abbreviations

kb:

kilobase(s)

bp:

base pairs

Ap:

ampicillin

Tc:

tetracycline

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Authors and Affiliations

  1. Division of Molecular Genetics, Institute for Molecular and Cellular Biology, Osaka University, Kita-ku, 530, Osaka, Japan

    Tomoaki Murotsu, Hiroshi Tsutsui & Kenichi Matsubara

Authors
  1. Tomoaki Murotsu
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  2. Hiroshi Tsutsui
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  3. Kenichi Matsubara
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Communicated by M. Takanami

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Murotsu, T., Tsutsui, H. & Matsubara, K. Identification of the minimal essential region for the replication origin of miniF plasmid. Mol Gen Genet 196, 373–378 (1984). https://doi.org/10.1007/BF00328075

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  • DOI: https://doi.org/10.1007/BF00328075

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