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The endolymphatic duct and sac of the rat: a histological, ultrastructural, and immunocytochemical investigation

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Abstract

A study of the ultrastructure, vascularization, and innervation of the endolymphatic duct and sac of the rat has been performed by means of light- and electron-microscopic and immunocytochemical methods. Two different types of epithelial cells have been identified: the ribosome-rich cell and the mitochondria-rich cell. These two cell types make up the epithelium of the complete endolymphatic duct and sac, although differences in their quantitative distribution exist. The morphology of the ribosome-rich cells varies between the different parts of the endolymphatic duct and sac; the morphology of the mitochondria-rich cells remains constant. According to the epithelial composition, vascularization, and structural organization of the lamina propria, both duct and sac are subdivided into three different parts. A graphic reconstruction of the vascular network supplying the endolymphatic duct and sac shows that the vascular pattern varies among the different parts. In addition, the capillaries of the duct are of the continuous type, whereas those supplying the sac are of the fenestrated type. Nerve fibers do not occur within the epithelium of the endolymphatic duct and sac. A few nerve fibers regularly occur in the subepithelial compartment close to the blood vessels; these fibers have been demonstrated in whole-mount preparations by the application of the neuronal marker protein gene product 9.5. Single beaded fibers immunoreactive to substance P and calcitonin-gene related peptide are observed within the same compartment. Dopamine-β-hydroxylase-immunoreactive axons are restricted to the walls of arterioles. Morphological differences between the different portions of the endolymphatic duct and sac are discussed with regard to possible roles in fluid absorption and immunocompetence.

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Abbreviations

CGRP:

Calcitonin gene-related peptide

DSP:

distal sac portion

DβH:

dopamine-β-hydroxylase

ED:

endolymphatic duct

ES:

endolymphatic sac

EDP:

enlarged duct portion

IR:

immunoreactive

ISP:

intermediate sac portion

LIS:

lateral intercellular space

NDP:

narrow duct portion

PMA:

posterior meningeal artery

PVA:

posterior vestibular artery

PGP 9.5:

protein gene product 9.5

PSP:

proximal sac portion

SP:

substance P

TDP:

transitional duct portion

VVA:

vem of vestibular aqueduct

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  1. Anatomisches Institut, Abteilung für Neuroanatomie der Ruhr-Universität Bochum, Universitätsstrasse 150, D-44801, Bochum, Germany

    Annegret Dahlmann & Monika von Düring

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  1. Annegret Dahlmann
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  2. Monika von Düring
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Dahlmann, A., von Düring, M. The endolymphatic duct and sac of the rat: a histological, ultrastructural, and immunocytochemical investigation. Cell Tissue Res 282, 277–289 (1995). https://doi.org/10.1007/BF00319118

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  • DOI: https://doi.org/10.1007/BF00319118

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