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The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals

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Summary

A multi-copy plasmid containing the SNQ3 gene confers hyper-resistance to 4-nitroquinoline-N-oxide (4NQO), Trenimon, MNNG, cycloheximide, and to sulfometuron methyl in yeast transformants. Restriction analysis, subcloning, and DNA sequencing revealed an open reading frame of 1950 bp on the SNQ3-containing insert DNA. Gene disruption and transplacement into chromosomal DNA yielded 4NQO-sensitive null mutants which were also more sensitive than the wild-type to Trenimon, cycloheximide, sulfometuron methyl, and MNNG. Hydropathic analysis showed that the SNQ3-encoded protein is most likely not membrane-bound, while the codon bias index points to low expression of the gene.

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Authors and Affiliations

  1. Institut für Mikrobiologie der J.W. Goethe-Universität, Theodor-Stern-Kai 7, Haus 75, W-6000, Frankfurt am Main, Germany

    Karin Hertle, Eckard Haase & Martin Brendel

Authors
  1. Karin Hertle
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  2. Eckard Haase
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  3. Martin Brendel
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Communicated by K. Wolf

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Hertle, K., Haase, E. & Brendel, M. The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals. Curr Genet 19, 429–433 (1991). https://doi.org/10.1007/BF00312733

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  • DOI: https://doi.org/10.1007/BF00312733

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