Summary
This study reports the histological, ultrastructural, and immunocytochemical characteristics of intracytoplasmic eosinophilic inclusion bodies occurring in various types of neurons of the human central nervous system. By light microscopy, the inclusions were brightly eosinophilic, slightly birefringent, and sharply demarcated; they were found in the thalamus in 92% of the cases, in the substantia nigra in 88%, in the locus coeruleus in 45%, and rarely in the spinal cord. Ultrastructurally, the inclusions were composed of assemblies of parallel, alternating dark and light rectilinear profiles. The dark profiles corresponded to thin filaments (microfilaments) that measured 5.5–6.0 nm in diameter. A second set of dense lines crisscrossed the first at right angles. In sections perpendicular to their long axis, the filaments were distributed in a tetragonal lattice pattern in which individual elements occupied the angles of a square. Immunocytochemical preparations for actin were negative. Due to their high rate of occurrence in nonpathological brains, it is thought that the inclusions represent a normal but as yet unidentified cytoplasmic organelle.
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References
Culebras A, Feldman RG, Merk FB (1973) Cytoplasmic inclusion bodies within neurons of the thalamus in myotonic dystrophy. A light and electron microscope study. J Neurol Sci 19:319–329
Culebras A, Segarra JM, Feldman RG (1972) Eosinophilic bodies within neurons in the human thalamus. An age-related histological feature. J Neurol Sci 16:177–182
Duffy PE, Tennyson VM (1965) Phase and electron microscopic observations of Lewy bodies and melanin granules in the substantia nigra and locus coeruleus in Parkinson's disease. J Neuropathol Exp Neurol 24:398–414
Fraser H (1969) Eosinophilic bodies in some neurones in the thalamus of ageing mice. J Pathol 98:201–204
Fraser H, Smith W, Gray EW (1970) Ultrastructural morphology of cytoplasmic inclusions within neurons of ageing mice. J Neurol Sci 11:123–127
Galloway PG, Perry G, Gambetti P (1987) Hirano body filaments contain actin and actin-associated proteins. J Neuropathol Exp Neurol 46:185–199
Goldman JE (1983) The association of actin with Hirano bodies. J Neuropathol Exp Neurol 42:146–152
Goldman JE (1986) Cytoskeletal constituents of Hirano bodies. J Neuropathol Exp Neurol 45:335 [abstr]
Hart MN, Cancilla PA, Frommes S, Hirano A (1977) Anterior horn cell degeneration and Bunina-type inclusions associated with dementia. Acta Neuropathol (Berl) 38: 225–228
Hirano A, Llena JF, Streifler M, Cohn DF (1976) Anterior horn cell changes in a case of neurolathyrism. Acta Neuropathol (Berl) 35:277–283
Kalnins VI (1978) The fibre systems of cells. Bull Microsc Soc Can 6:4–11
Morecki R, Zimmerman HM (1969) Human rabies encephalitis. Fine structure study of cytoplasmic inclusions. Arch Neurol 20:599–604
Ogata J, Budzilovich GN, Cravioto H (1972) A study of rod-like structures (Hirano bodies) in 240 normal and pathological brains. Acta Neuropathol (Berl) 21:61–67
Ono S, Inoue K, Mannen T, Kanda F, Jinnai K, Takahashi K (1987) Neuropathological changes of the brain in myotonic dystrophy — some new observations. J Neurol Sci 81:301–320
Pena CE (1980) Intracytoplasmic neuronal inclusions in the human thalamus. Light microscopic, histochemical, and ultrastructural observations. Acta Neuropathol (Berl) 52: 157–159
Peña CE (1981) Intracytoplasmic neuronal inclusions in the human central nervous system. An unrecognized form of neuronal protein storage. Light microscopic, histochemical and ultrastructural study. J Neuropathol Exp Neurol 40:311 [abstr]
Schochet SS (1972) Neuronal inclusions. In: Bourne GH (ed) The structure and function of nervous tissue, vol 4. Academic Press, New York, pp 129–177
Schochet SS, McCormick WF (1972) Ultrastructure of Hirano bodies. Acta Neuropathol (Berl) 21:50–60
Schochet SS, Wyatt RB, McCormick WF (1970) Intracytoplasmic acidophilic granules in the substantia nigra. Arch Neurol 22:550–555
Shelanski ML, Wisniewski H (1969) Neurofibrillary degeneration induced by vincristine therapy. Arch Neurol 20:199–206
Slyter H, Liwnicz B, Herrick MK, Mason R (1980) Fatal myeloencephalopathy caused by intrathecal vincristine. Neurology 30:867–871
Takei Y, Mirra SS (1971) Intracytoplasmic hyaline inclusion bodies in the nerve cells of the hypoglossal nucleus in human autopsy material. Acta Neuropathol (Berl) 17:14–23
Tomonaga M (1974) Ultrastructure of Hirano bodies. Acta Neuropathol (Berl) 28:365–366
Tomonaga M, Saito M, Yoshimura M, Shimada H, Tohgi H (1978) Ultrastructure of the Bunina bodies in anterior horn cells of amyotrophic lateral sclerosis. Acta Neuropathol (Berl) 42:81–86
Wisniewski HM, Berry K, Spiro AJ (1975) Ultrastructure of thalamic neuronal inclusions in myotonic dystrophy. J Neurol Sci 24:321–329
Wisotzkey HM, Moossy J (1972) Hyaline (“colloid”) cytoplasmic inclusions in neurons of human hypoglossal nuclei. Arch Pathol 93:61–70
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Peña, C.E., Katoh, A. Intracytoplasmic eosinophilic inclusions in the neurons of the central nervous system. Acta Neuropathol 79, 73–77 (1989). https://doi.org/10.1007/BF00308960
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DOI: https://doi.org/10.1007/BF00308960