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Protein staining of ribboned epon sections for light microscopy

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Summary

Procedures are described for obtaining and handling ribboned epon sections 0.3–2 μ thick for light microscopy, and for the cytological application of two intense acid dyes, Aniline Blue Black and Coomassie Brilliant Blue R 250. The technique allows precise localization of proteins and some other materials, and, because the sections are ribboned, facilitates three-dimensional visualization of the structures involved. The dyes may be used in combination with the periodic acid-Schiff reaction and with autoradiography.

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Author notes

  1. Donald B. Fisher

    Present address: Department of Botany, University of Georgia, 30601, Athens, Georgia

Authors and Affiliations

  1. Department of Botany, University of California, 94720, Berkeley, California

    Donald B. Fisher

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  1. Donald B. Fisher
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Additional information

This work was supported in part by a Public Health Service fellowship 5-F2-GM-22, 031-02 to the author and in part by NSF grant GB 3460 to Dr. W. A. Jensen.

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Fisher, D.B. Protein staining of ribboned epon sections for light microscopy. Histochemie 16, 92–96 (1968). https://doi.org/10.1007/BF00306214

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  • DOI: https://doi.org/10.1007/BF00306214

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