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Microbial sensor for aspartame

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Summary

A microbial amperometric sensor using immobilized Bacillus subtilis cells was developed for the determination of the dipeptide sweetener aspartame (l-aspartyl-l-phenylalaninemethylester). From 0.07 to 0.6 mmol/l aspartame, a linear dependence of the initial current change (i.e., change in respiration rate) was obtained. The sensitivity for aspartame was one order of magnitude higher than for its amino acid constituents. The microbial sensor was stable for 8 weeks.

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Authors and Affiliations

  1. Central Institute of Molecular Biology, Department of Applied Enzymology, Academy of Sciences of the GDR, Robert-Rössle-Str. 10, 1115, Berlin-Buch, German Democratic Republic

    R. Renneberg, K. Riedel & F. Scheller

Authors
  1. R. Renneberg
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  2. K. Riedel
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  3. F. Scheller
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Renneberg, R., Riedel, K. & Scheller, F. Microbial sensor for aspartame. Appl Microbiol Biotechnol 21, 180–181 (1985). https://doi.org/10.1007/BF00295116

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  • DOI: https://doi.org/10.1007/BF00295116

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