Abstract
Evidence is presented that chromosome ends are attached to the nuclear envelope prior to the formation of axial cores during early meiotic prophase in the grasshopper Brachystola magna. The attachment sites of distal and proximal chromosome ends are clustered in a small region of the inner nuclear envelope resulting in a classical bouquet arrangement of the chromosomes. Proximal ends are tightly clustered due to the presence of chromocenters. Distal chromosome ends are more widely scattered throughout the base of the bouquet. —Axial core formation can be initiated at chromosome ends or at internal chromosome sites. However, there is a preference for axial cores to form in distal chromosome regions rather than proximal regions during early meiotic prophase. — Virtually all of the nuclear pore complexes are located in the general vicinity of the chromosome attachment sites but each specific attachment site is surrounded by a small area of nuclear envelope which is devoid of pore complexes.
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References
Baker, T.G., Franchi, L.L.: The fine structure of oogonia and oocytes in human ovaries. J. Cell. Sci. 2, 213–224 (1967)
Becak, M.L., Becak, W.: Origin of the synaptonemal complex. Experientia (Basel 28, 1367–1369 (1972)
Carpenter, A.T.C.: Electron microscopy of meiosis in Drosophila melanogaster females. 1 Structure, arrangement, and temporal change of the synaptonemal complex in wild type. Chromosoma (Berl.) 54, 157–182 (1975)
Church, K., Moens, P.B.: Centromere behavior during interphase and meiotic prophase in Allium fistulosum from 3-D, E.M. reconstruction. Chromosoma (Berl.) 56, 249–263 (1976)
Comings, D.E., Okada, T.A.: Whole mount electron microscopy of meiotic chromosomes and the synaptonemal complex. Chromosoma (Berl.) 30, 269–286 (1970)
Counce, S.J., Meyer, G.F.: Differentiation of the synaptonemal complex and kinetochore in Locusta spermatocytes studied by whole mount electron microscopy. Chromosoma (Berl.) 44, 231–253 (1973)
Darlington, C.D.: Recent advances in cytology, 2nd edit. London: Churchill 1937
Driscoll, C.J., Darvey, N.L.: Nucleolar behaviour in triticum. Chromosoma (Berl.) 36, 131–139 (1972)
Engelhardt, P., Pusa, K.: Nuclear pore complexes: “press-stud” elements of chromosomes in pairing and control. Nature (Lond.) New Biol. 240, 163–166 (1972)
Esponda, P., Giménez-Martin, G.: The attachment of the synaptonemal complex to the nuclear envelope. An Ultrastructural and Cytochemical Analyses. Chromosoma (Berl.) 38, 405–417 (1972)
Fiil, A., Moens, P.B.: The development, structure and function of modified synaptonemal complexes in mosquito oocytes. Chromosoma (Berl.) 41, 37–62 (1973)
Gillies, C.B.: An ultrastructural analysis of chromosome pairing in maize. C.R. Trav. Lab. Carlsberg 40, 135–161 (1975a)
Gillies, C.B.: Synaptonemal complex and chromosome structure. Ann. Rev. Genet. 9, 91–109 (1975b)
Hsu, T.C., Cooper, J.E.K., Mace, M.L., Brinkley, B.R.: Arrangement of centromeres in mouse cells. Chromosoma (Berl.) 34, 73–87 (1971)
Jones, G.H.: Modified synaptonemal complexes in spermatocytes of Stethophyma grossum. C.S.H.S. 38, 109–115 (1973)
Kasha, K.J., Burnham, C.R.: The location of interchange breakpoints in barley. II. Canad. J. Genet. Cytol. 7, 620–632 (1965)
LaCour, L.F., Wells, B.: Chromocenters and the synaptonemal complex. J. Cell Sci. 6, 665–667 (1970)
Maguire, M.P.: Evidence for homologous pairing prior to meiotic prophase in maize. Chromosoma (Berl.) 21, 221–231 (1967)
MacGregor, H.C., Kezer, J.: The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus. Chromosoma (Berl.) 33, 167–182 (1971)
Moens, P.B.: The fine structure of meiotic chromosome polarization and pairing in Locusta migratoria spermatocytes. Chromosoma (Berl.) 28, 1–25 (1969)
Moens, P.B.: Serial sectioning in electron microscopy. Proc. Canad. Fed. Biol. Soc. 13, 160 (1970)
Moens, P.B.: Quantitative electron microscopy of chromosome organization at meiotic prophase. C.S.H.S. 38, 99–107 (1973)
Moses, M.J.: Synaptinemal complex. Ann. Rev. Genet. 2, 263–412 (1968)
Rasmussen, S.V.: Studies on the development of the synaptonemal complex in Drosophila melanogaster. C.R. Trav. Lab. Carlsberg 39, 443–468 (1974)
Rasmussen, S.V.: The meiotic prophase in Bombyx mori females analyzed by three-dimensional reconstructions of synaptonemal complexes. Chromosoma (Berl.) 54, 245–293 (1976)
Rickards, G.K.: Prophase chromosome movements in living house cricket spermatocytes and their relationship to prometaphase, anaphase and granule movements. Chromosoma (Berl.) 49, 407–445 (1975)
Westergard, M., Wettstein, D. v.: The synaptinemal complex. Ann. Rev. Genet. 6, 71–110 (1972)
Wettstein, R., Sotelo, R.J.: The molecular architecture of the synaptonemal complexes. Advanc. Cell molec. Biol. 1, 109–152 (1971)
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Church, K. Arrangement of chromosome ends and axial core formation during early meiotic prophase in the male grasshopper Brachystola magna by 3D, E.M. reconstruction. Chromosoma 58, 365–376 (1976). https://doi.org/10.1007/BF00292844
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DOI: https://doi.org/10.1007/BF00292844