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Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids

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Abstract

Transcription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction.

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Author notes

  1. Mathilde Grelon, Françoise Budar, Sandrine Bonhomme & Georges Pelletier

    Present address: Station de Génétique et d'Amélioration des Plantes, INRA centre de Versailles, 78026, Versailles cedex, France

Authors and Affiliations

  1. Laboratoire de Biologie cellulaire, INRA centre de Versailles, 78026, Versailles cedex, France

    Mathilde Grelon, Françoise Budar, Sandrine Bonhomme & Georges Pelletier

Authors
  1. Mathilde Grelon
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  2. Françoise Budar
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  3. Sandrine Bonhomme
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  4. Georges Pelletier
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Communicated by D. M. Lonsdale

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Grelon, M., Budar, F., Bonhomme, S. et al. Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids. Molec. Gen. Genet. 243, 540–547 (1994). https://doi.org/10.1007/BF00284202

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  • DOI: https://doi.org/10.1007/BF00284202

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