Summary
Plasmid pIP173, isolated from Salmonella ordonez strain BM2000, confers resistance to tetracycline and a number of other antibiotics. We determined the nucleotide sequence of the pIP173 tetR repressor and tetA resistance genes. The pIP173 tetR gene is essentially identical to the class D tetR gene from plasmid RA1. The pIP173 tet genes are flanked by directly repeated copies of the insertion sequence IS26. Interestingly, the 3′ end of the tetR gene, encoding the C-terminal 16 amino acids of the TetR protein, extends into the flanking IS26 sequence. The relationships between the class A, B, C, and D TetA sequences parallel the relationships between the corresponding TetR sequences; class D is more closely related to class B than to either class A or C. Overall, the four TetA sequences show 38% identity and 57% similarity.
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Communicated by J.W. Lengeler
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Allard, J.D., Gibson, M.L., Vu, L.H. et al. Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez . Molec. Gen. Genet. 237, 301–305 (1993). https://doi.org/10.1007/BF00282811
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DOI: https://doi.org/10.1007/BF00282811