Summary
We describe a genomic DNA segment from spinach that bears part of the single-copy gene for ferredoxin-NADP+-oxidoreductase (FNR) including a 3.4 kb promoter sequence. Dissection of this DNA segment and its analysis in GUS (β-glucuronidase) gene fusions in transgenic tobacco demonstrated that the promoter differs in structure from all other promoters for thylakoid protein genes studied to date. Two regions with light-responsive elements were identified. One is located within the first 118 by upstream of the transcription initiation site. A second fragment covering nucleotide positions − 220 to − 119 is capable of conferring light-dependent GUS gene expression on two different minimal promoters. The latter fragment binds a trans-acting factor in gel-shift assays. None of the fragments carries cis elements known from other genes to be involved in light-controlled expression. Comparison of the light responsiveness of GUS gene fusions controlled by the −753/+231 and −118/+231 regions indicates that they respond differentially to phytochrome-dependent signals and that their expression in tobacco is not restricted to tissue with functional chloroplasts.
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Communicated by R. Hagemann
The sequence data reported here appear in the EMBL data library under the accession numbers X64351 (FNR) and X64350 (cab)
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Oelmüller, R., Bolle, C., Tyagi, A.K. et al. Characterization of the promoter from the single-copy gene encoding ferredoxin-NADP+-oxidoreductase from spinach. Molec. Gen. Genet. 237, 261–272 (1993). https://doi.org/10.1007/BF00282808
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DOI: https://doi.org/10.1007/BF00282808