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Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication

An improved fixation procedure for electron microscopy of plant and animal cells

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Summary

A fixation procedure for electron microscopy is described which includes a simultaneous glutaraldehyde-OsO4 fixation followed by postosmication. This procedure was found to have considerable advantages in preserving structures of plant and animal cells.

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Author information

Authors and Affiliations

  1. Division of Cell Biology, Institute of Biology II, University of Freiburg i. Br., Germany

    Werner W. Franke, Sigrid Krien & R. Malcolm Brown Jr.

  2. Department of Botany, University of North Carolina, 27514, Chapel Hill, N.C., USA

    Werner W. Franke, Sigrid Krien & R. Malcolm Brown Jr.

Authors
  1. Werner W. Franke
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  2. Sigrid Krien
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  3. R. Malcolm Brown Jr.
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Visiting NSF postdoctoral fellow.

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Franke, W.W., Krien, S. & Brown, R.M. Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication. Histochemie 19, 162–164 (1969). https://doi.org/10.1007/BF00281096

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  • DOI: https://doi.org/10.1007/BF00281096

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