Skip to main content
SpringerLink
Log in

On the nature of β-galactosidase synthesized by the DNA-directed cell-free system

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The β-galactosidase product of the DNA-directed cell-free system for the synthesis of protein of the lac operon, developed by Zubay and his colleagues, has been purified to radioactive homogeneity and compared to wild-type β-galactosidase. When analyzed by sucrose gradient centrifugation, SDS-gel electrophoresis, and kinetic analysis, the purified cell-free enzyme behaves identically to the purified wild-type β-galactosidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chambers, D. A., Zubay, G.: The stimulatory effect of cyclic adenosine 3′5′ monophosphate on DNA-directed synthesis of β-galactosidase in a cell-free system. Proc. nat. Acad. Sci. (Wash.) 63, 118 (1969).

    Google Scholar 

  • Craven, G. R., Steers, E., Anfinsen, C. B.: Purification, composition and molecular weight of the β-galactosidase of Escherichia coli K12. J. biol. Chem. 240, 2468 (1965).

    Google Scholar 

  • DeVries, J. K., Zubay, G.: Characterization of a β-galactosidase formed between a complementary protein and a peptide synthesized De Novo. J. Bact. 97, 1419 (1969).

    Google Scholar 

  • Hamlin, J., Zabin, I.: β-galactosidase: Immunological activity of ribosomal-bound growing peptide chains. Proc. nat. Acad. Sci. (Wash.) 69, 412 (1972).

    Google Scholar 

  • Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Amer. chem. Soc. 56, 658 (1934).

    Google Scholar 

  • Reid, M. S., Bieleski, R. L.: A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis. Analyt. Biochem. 22, 374 (1968).

    Google Scholar 

  • Waller, J. P.: The NH2-terminal peptides of the proteins from cell-free extracts of E. coli. J. molec. biol. 7, 483 (1963).

    Google Scholar 

  • Weber, K., Osborn, M.: The reliability of molecular weight determination by dodecyl sulfatepolyacrylamide gel electrophoresis. J. biol. Chem. 244, 4406 (1969).

    Google Scholar 

  • Zubay, G., Chambers, D. A.: A DNA-directed cell-free system for β-galactosidase synthesis; Characterization of the De Novo synthesized enzyme and some aspects of the regulation of synthesis. Cold Spr. Harber. Symp. quant. Biol. 34, 753 (1969).

    Google Scholar 

  • Zubay, G., Chambers, D. A., Cheong, L. C.: Cell-free studies on the regulation of the lac operon, in The Lactose Operon (ed. J. R. Beckwith and D. Zipser), Cold Spr. Harb. Lab. quant. Biol. p. 375 (1970).

  • Zubay, G., Cheong, L., Gefter, M.: The DNA-directed cell-free synthesis of a biologically active transfer. RNA. Proc. nat. Acad. Sci. (Wash.) 68, 2195 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, USA

    D. A. Chambers & J. L. Manley

Authors
  1. D. A. Chambers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. L. Manley
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by E. Bautz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chambers, D.A., Manley, J.L. On the nature of β-galactosidase synthesized by the DNA-directed cell-free system. Molec. Gen. Genet. 120, 301–308 (1973). https://doi.org/10.1007/BF00268144

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268144

Keywords

Search

Navigation