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Early and late transfer of F genes by Hfr donors of E. coli K-12

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Summary

The results of short interrupted matings between an Hfr donor and a recipient strain carrying a temperature-sensitive replication mutant (frp −) of Flac demonstrate that the Hfr strain transfers this frp gene of F early in conjugation. This frp gene was also shown to function in the maintenance of mutant F′ plasmids which appear to be generated from the DNA transferred early in conjugation by Hfr donors. In the course of these experiments, it was further demonstrated that certain Hfr strains which had been described as transferring the tra genes early in fact transfer that region of F late in conjugation.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, 20014, Bethesda, Marland, USA

    Mark S. Guyer

  2. Department of Molecular Biology, University of California, 94720, Berkeley, California, USA

    Alvin J. Clark

Authors
  1. Mark S. Guyer
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  2. Alvin J. Clark
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Communicated by H.W. Boyer

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Guyer, M.S., Clark, A.J. Early and late transfer of F genes by Hfr donors of E. coli K-12. Molec. Gen. Genet. 157, 215–222 (1977). https://doi.org/10.1007/BF00267400

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  • DOI: https://doi.org/10.1007/BF00267400

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