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Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells

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Summary

The plasmid designated pAD1 was isolated from the cells of four variants of Bacillus brevis var. G.-B. The plasmid DNA has a molecular weight of about 47.1×106 daltons and contains 43.4 mole % G+C. The bulk of pAD1 DNA (96–98%) is associated with the fraction of chromosome DNA and membranes.

Restriction endonucleases SmaI, SalI and BamHI cleaved the plasmid DNA into two, two and six fragments, respectively. The cleavage map of the pAD1 genome has been constructed for these three endonucleases. Restriction enzymes EcoRI, HindIII, KpnI and PstI hydrolized the plasmid DNA into 16, 21, 10 and 9 fragments, respectively. The presence of repeated sequences in the plasmid genome was shown based on pAD1 DNA cleavage by these endonucleases.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics of Microorganisms Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, 142292, Pushchino on Oka, Moscow region, USSR

    A. P. Dobritsa, Svetlana V. Dobritsa & V. I. Tanyashin

  2. Microbial Ontogenesis Research Unit, Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, 142292, Pushchino on Oka, Moscow region, USSR

    A. P. Dobritsa, Svetlana V. Dobritsa & V. I. Tanyashin

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  1. A. P. Dobritsa
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  2. Svetlana V. Dobritsa
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  3. V. I. Tanyashin
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Communicated by D. Goldfarb

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Dobritsa, A.P., Dobritsa, S.V. & Tanyashin, V.I. Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells. Molec. Gen. Genet. 164, 195–204 (1978). https://doi.org/10.1007/BF00267384

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  • DOI: https://doi.org/10.1007/BF00267384

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