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Isolation and characterization of cDNA clones encoding Photosystem I subunits with molecular masses 11.0, 10.0 and 8.4 kDa from Chlamydomonas reinhardtii

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Summary

cDNA clones encoding three photosystem I subunits of Chlamydomonas reinhardtii with apparent molecular masses 13, 5 and 3 kDa (thylakoid polypeptides 28, 35 and 37; P28, P35 and P37, respectively) were isolated using gene specific oligonucleotides as probes. The sequences of these oligonucleotides were deduced from the N-terminal amino acid sequences of the proteins. The cDNAs were sequenced and used to probe Southern and Northern blots. The Southern blot analysis indicates that the proteins are encoded by single-copy genes. The mRNA sizes of the three components are 960 (P28), 1120 (P35) and 790 (P37) nucleotides. Comparison between the open reading frames of the cDNAs and the N-terminal amino acid sequences of the proteins indicates that the nascent polypeptides possess N-terminal transit sequences that are removed to give mature proteins of 11.0 (P28), 10.0 (P35) and 8.4 (P37) kDa. Analysis of the deduced protein sequences suggests that P28 and P35 are extrinsic membrane proteins and that P37 spans the thylakoid membrane. All three proteins have short transit peptides that probably route them to the stromal side of the thylakoid membrane.

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Abbreviations

OEE1, 2 and 3:

oxygen evolution enhancer proteins 1, 2 and 3

RuBisCO:

ribulose bisphosphate carboxylase/oxygenase

PS:

photosystem

P28, P35 and P37:

Chlamydomonas reinhardtii thylakoid polypeptides 28, 35 and 37

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Authors and Affiliations

  1. Departments of Molecular Biology and Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland

    Lars-Gunnar Franzén & Jean-David Rochaix

  2. Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule, Hönggerberg, CH-8093, Zürich, Switzerland

    Gerhard Frank & Herbert Zuber

Authors
  1. Lars-Gunnar Franzén
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  2. Gerhard Frank
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  3. Herbert Zuber
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  4. Jean-David Rochaix
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Additional information

Communicated by R.G. Herrmann

The nucleotide sequences presented here will appear in the EMBL/Genbank/DDBJ Nucleotide Sequence Databases under the accession numbers X15164 (11.0 kDa subunit; P28), X15165 (10.0 kDa subunit; P35) and X15166 (8.4 kDa subunit; P37)

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Franzén, LG., Frank, G., Zuber, H. et al. Isolation and characterization of cDNA clones encoding Photosystem I subunits with molecular masses 11.0, 10.0 and 8.4 kDa from Chlamydomonas reinhardtii . Molec. Gen. Genet. 219, 137–144 (1989). https://doi.org/10.1007/BF00261169

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  • DOI: https://doi.org/10.1007/BF00261169

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