Skip to main content
SpringerLink
Log in

Post-castration rebound of an androgen regulated prostatic gene

  • Original Article
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

After castration, the rat dorsolateral prostate M-40 mRNA initially decreased then rebounded to precastrated levels. The cellular site of M-40 expression and its renewed expression after castration was defined by in situ hybridization histochemistry. In situ hybridization with either a 32P-labeled or biotin-labeled M-40 cDNA probe demonstrated that M-40 mRNA levels were higher in the lateral than dorsal prostate. A second androgen regulated gene, RWB, also was highly expressed in the lateral prostate. The biotinylated cDNA probes provided microscopic resolution of the expressing cells, revealing two distinct morphologies of lateral epithelium which expressed both the M-40 and RWB mRNA. These morphologies appeared in ducts which contained either epithelial cell sheets that were highly convoluted or thinner epithelial cells with a minimal degree of convolution. The RWB mRNA decreased in both cell populations in response to androgen withdrawal. The decline and reappearance of M-40 mRNA also appeared in both epithelial cell types. These data demonstrated that after castration the M-40 mRNA initially decreased as expected for an androgen sensitive gene and then progressed to a fully inducible state. The mechanism of this progression remains to be elucidated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Parker MG, White R, Williams JG: Cloning and characterization of androgen-dependent mRNA from rat ventral prostate. J Biol Chem 255: 6996–7001, 1980

    Google Scholar 

  2. Carter DB, Yamada K, Harris SE: Developmental aspects of androgen-dependent mRNA from rat ventral prostate using cloned cDNA. Mole and Cell Endocr 31: 199–214, 1983

    Google Scholar 

  3. Gunn SA, Gould TC: A correlative anatomical and functional study of the dorsolateral prostate of the rat. Anat Rec 128: 41–53, 1957

    Google Scholar 

  4. Gunn SA, Gould TC, Genorl SS, Morse JG: Selective uptake of Zn65 by the dorsolateral prostate of the rat. Proc Soc Exp Biol and Med 88: 556–558, 1955

    Google Scholar 

  5. Schrodt GR: The fine structure of the lateral lobe of the rat prostate gland. J Ultrastructure Res 5: 485–496, 1961

    Google Scholar 

  6. Cunha GR, Donjacour AA, Cooke PS, Mee S, Bigsby RM, Higgins SJ, Sugimura Y The endocrinology and developmental biology of the prostate. Endocrine Reviews 8: 338–362, 1987

    Google Scholar 

  7. Dodd JG, Sheppard PC, Matusik RJ: Characterization and cloning of rat dorsal prostate mRNAs. J Biol Chem 258: 10731–10737, 1983

    Google Scholar 

  8. Dodd JG, Kreis C, Sheppard PC, Hamel A, Matusik RJ: Effects on androgens on mRNA for a secretory protein of rat dorsolateral prostate and seminal vesicles. Mole and Cell Endoer 47: 191–200, 1986

    Google Scholar 

  9. Ho K-C, Wilson EM, French FS: Androgen regulated prostate genes: structural analysis and regulation. In: Coffey DS, Bruchovsky N, Gardner Jr WA, Resnick MI, Karr JP (eds) Current Concepts and Approaches to The Study of Prostate Cancer. AR Liss Inc., New York, 1987, pp 125–154

    Google Scholar 

  10. Gee CE, Roberts JC: In situ hybridization histochemistry: A technique for the study of gene expression in single cells. DNA 2: 157–163, 1983

    Google Scholar 

  11. Singer RH, Ward DC: Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog. Proc Natl Acad Sci (USA) 79: 7331–7335, 1982

    Google Scholar 

  12. Coffey DS: The effects of androgens on DNA and RNA synthesis in sex accessory sex tissue. In: Brandes D (ed) Male Accessory Organs; Structure and Function in Mammals. Academic Press, New York, 1974, pp 307–328

    Google Scholar 

  13. Matusik RJ, Kreis C, McNicol P, Sweetland R, Mullin C, Fleming WH, Dodd JG: Regulation of prostatic genes: role of androgens and zinc in gene expression. Biochem Cell Biol 64: 601–607, 1986

    Google Scholar 

  14. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ: Isolation of active ribonucleic acids from sources enriched in ribonuclease. Biochemistry 18: 5294–5298, 1979

    CAS  PubMed  Google Scholar 

  15. White BA, Bancroft FC: Cytoplasmic dot hybridization. J Biol Chem 257: 8569–8572, 1982

    Google Scholar 

  16. Hoskina M, Boothbry M, Boime I: Cytological localization of chorionic gonadotropin and placental lactogen mRNA during development of the human placenta. J Cell Biol 93: 190–198, 1982

    Google Scholar 

  17. Duckworth ML, Kirk KL, Friesen HG: Isolation and identification of a cDNA clone of rat placental lactogen II. J Biol Chem 261: 10871–10878, 1986

    Google Scholar 

  18. Brandes D: The fine structure and histochemistry of prostatic glands in relation to sex hormones. Int Rev Cytol 20: 207–276, 1966

    Google Scholar 

  19. Donjacour AA, Cunha GO, Sugimura Y Heterogeneity of structure and function in the mouse prostate. In: Coffey DS, Bruchovsky W, Gardner Jr WA, Resnick MI, Kars JP (eds) Current Concepts and Approaches to The Study of Prostate Cancer. AR Liss Inc., New York, 1987, pp 583–600

    Google Scholar 

  20. Wilson EM, French FS: Biochemical homology between rat dorsal prostate and coagulating gland. J Biol Chem 255: 10946–10953, 1980

    Google Scholar 

  21. Page MJ, Parker MG: Effect of androgens on the transcription of rat prostatic binding protein genes. Mole Cell End 27: 343–355, 1982

    Google Scholar 

  22. Zhang YL, Parker MG: Regulation of prostatic steroid binding proteins mRNAs by testosterone. Mole Cell End 43: 151–154, 1985

    Google Scholar 

  23. Quarmby VE, Beckman Jr WC, Wilson EM, French FS: Androgen regulation of c-myc messenger ribonucleic acid levels in rat ventral prostate. Molecular Endocrin 1: 865–874, 1987

    Google Scholar 

  24. Saltzmann AG, Hiipakka RA, Chang C, Liao SJ: Androgen repression of the production of a 29-kilodalton protein and its mRNA in the rat ventral prostate. J Biol Chem 262: 432–437, 1987

    Google Scholar 

  25. Leger JG, Montpetit ML, Tenniswood MP: Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate. Biochem Biophys Res Commun 147: 196–203, 1987

    Google Scholar 

  26. Traish AM, Wotiz HH: Prostatic epidermal growth factor receptor and their regulation by androgen. Endocrinology 121: 1261–1267, 1987

    Google Scholar 

  27. Darbre PD, King RJB: Progression to steroid insensitivity can occur irrespective of the presence of functional steroid receptors. Cell 51: 521–528, 1987

    Google Scholar 

  28. Evans GS, Chandler JA: Cell proliferation studies in the rat prostate: II. The effects of castration and androgen-induced regeneration upon basal and secretory cell proliferation. The Prostate 11: 327–338, 1987

    Google Scholar 

  29. Timms BG, Chandler JA: Ultrastructural and analytical studies on the prostates of castrated rats. The Prostate 4: 37–55, 1983

    Google Scholar 

  30. Prins GS, Reiher J: Androgens differentially regulate nuclear androgen receptor levels in the ventral, dorsal and lateral prostate. The Endocrine Society: Abstract 792, 1987

  31. Kyprianou N, Isaacs JT Quantal relationship between prostatic dihydrotestosterone and prostatic cell content: critical threshold concept. The Prostate 11: 41–50, 1987

    Google Scholar 

  32. Prins GS: Prolactin influence on cytosol and nuclear androgen receptors in the ventral, dorsal and lateral lobes of the rat prostate. Endocrin 120: 1457–1464, 1987

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, University of Manitoba, R3E OW3, Winnipeg, Manitoba, Canada

    Robert Sweetland, Patricia C. Sheppard & Robert J. Matusik

  2. Second address Manitoba Institute of Cell Biology, R3E OW3, Manitoba, Canada

    Janice G. Dodd

Authors
  1. Robert Sweetland
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patricia C. Sheppard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Janice G. Dodd
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert J. Matusik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sweetland, R., Sheppard, P.C., Dodd, J.G. et al. Post-castration rebound of an androgen regulated prostatic gene. Mol Cell Biochem 84, 3–15 (1988). https://doi.org/10.1007/BF00235188

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00235188

Key words

Search

Navigation