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In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation

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Abstract

An efficient protocol has been developed for the in vitro propagation of Bambusa tulda through shoot proliferation. Shoots from 3-week-old aseptically grown seedlings were used to initiate cultures. Multiple shoots were obtained on liquid Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (8×10−6M) and kinetin (4×10−6M). Continuous shoot proliferation at a rate of 4–5 fold every three weeks was achieved through forced axillary branching. More than 90% of the shoots could be rooted on a modified MS medium containing indoleacetic acid (1×10−5M) and coumarin (6.8×10−5M). Following simple hardening procedures, the in vitro raised plants were transferred to the soil with more than 80% success.

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Abbreviations

BAP:

6-benzylaminopurine

2-ip:

6-γ,γ-dimethylallylaminopurine

Kn:

kinetin

IAA:

3-indoleacetic acid

IBA:

3-indolebutyric acid

NAA:

1-naphthaleneacetic acid

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Communicated by I. K. Vasil

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Saxena, S. In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Reports 9, 431–434 (1990). https://doi.org/10.1007/BF00232266

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  • DOI: https://doi.org/10.1007/BF00232266

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