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Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol

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Abstract

Pretreatment with increasing concentrations of mannitol, from 0.3 to 0.7 M, was used to induce stress in cultured anthers of barley (Hordeum vulgare L.). Three cultivars with varying degrees of androgenetic ability were studied. A positive linear relationship was found between concentration of mannitol in the pretreatment medium and the number of regenerated green doubled haploid plants in all the cultivars. The pretreatment also resulted in an increasing proportion of embryos to dividing microspores, and in green to albino plantlets. The optimum length of the pretreatment seemed to be genotype dependent. When Ficoll was used as an alternative stress agent a differential genotype response was observed.

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Abbreviations

BAP N6 :

benzyl-aminopurine

IAA:

indol acetic acid

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Authors and Affiliations

  1. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Apartado 202, E-50080, Zaragoza, Spain

    L. Cistué, A. Ramos & A. M. Castillo

  2. Centre UdL-IRTA, Av Rovira Roure 177, 25006, Lleida, Spain

    I. Romagosa

Authors
  1. L. Cistué
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  2. A. Ramos
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  3. A. M. Castillo
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  4. I. Romagosa
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Communicated by I. K. Vasil

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Cistué, L., Ramos, A., Castillo, A.M. et al. Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol. Plant Cell Reports 13, 709–712 (1994). https://doi.org/10.1007/BF00231629

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  • DOI: https://doi.org/10.1007/BF00231629

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