Abstract
A substantial level of xylose was detected in the pectic polysaccharides that had been extracted from carrot (Daucus carota L.) calli and purified by gel-permeation and ion-exchange chromatography. The results of the removal of neutral sugar chains and β-elimination indicated that the xylose was not included in the neutral sugar chains but was directly bound to a polygalac-turonic-acid backbone. Methylation analysis confirmed that the xylose was directly linked to galacturonic acid at position 2 or 3, as a terminal residue. The amount of xylose was positively correlated with the size of cell clusters in several lines of cultured carrot cells.
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Abbreviations
- EC:
-
embryogenic callus
- 4-GalA:
-
4-linked galacturonic acid
- NC:
-
non-embryogenic callus
- T-Xyl:
-
terminal xylose
- 3,4-GalA:
-
3,4-linked galacturonic acid
- 2,4-GalA:
-
2,4-linked galacturonic acid
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Part of this work was supported by a research grant from the Science and Technology Agency of Japan and a Grand-in-Aid from the Ministry of Education, Science and Culture, Japan. The authors are grateful to Dr. Koichi Kakegawa of the Forestry and Forest Products Research Institute for his encouragement throughout this research.
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Kikuchi, A., Edashige, Y., Ishii, T. et al. A xylogalacturonan whose level is dependent on the size of cell clusters is present in the pectin from cultured carrot cells. Planta 200, 369–372 (1996). https://doi.org/10.1007/BF00231391
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DOI: https://doi.org/10.1007/BF00231391