Summary
Heparin (5 U/ml) induced the release of LPL into the incubation medium of cardiac myocytes isolated from adult rat hearts. The secretion of LPL occurred in two phases: a rapid release (5–10 min of incubation with heparin) that was independent of protein synthesis followed by a slower rate of release that was inhibited by cycloheximide. The rapid release of LPL induced by heparin likely occurs from sites that are at or near the cell surface. LPL secretion could also be stimulated by heparan sulfate and dermatan sulfate, but not by hyaluronic acid, chondroitin sulfate or keratan sulfate. Heparin-releasable LPL activity measured in short-term incubations represented a large fraction (40–50%) of the initial LPL activity associated with myocytes, but the fall in cellular LPL activity following heparin was less than the amount of LPL activity secreted into the incubation medium. This discrepancy was not due to latency of LPL in the pre-heparin cell homogenates, but in part could be due to a three-fold greater affinity of the heparin-released enzyme for substrate as compared to LPL in post-heparin myocyte homogenates.
Similar content being viewed by others
Abbreviations
- LPL:
-
lipoprotein lipase
References
Cryer A: Tissue lipoprotein lipase activity and its action in lipoprotein metabolism. Int J Biochem 13:525–541, 1981
Hamosh M, Hamosh P: Lipoprotein lipase: its physiological and clinical significance. Molec Aspects Med 6:199–289, 1983
Pederson ME, Cohen M, Schotz MC: Immunocytochemical localization of the functional fraction of lipoprotein lipase in the perfused heart. J Lipid Res 24:512–521, 1983
Borensztajn J, Robinson DS: The effect of fasting on the utilization of chylomicron triglyceride fatty acids in relation to clearing factor lipase (lipoprotein lipase) releasable by heparin in the perfused rat heart. J Lipid Res 11:111–117, 1970
Schotz MC, Twu J-S, Pederson ME, Chen C-H, Garfinkel AS, Borensztajn J: Antibodies to lipoprotein lipase-application to perfused heart. Biochim Biophys Acta 489:214–224, 1977
Ben-Zeev O, Schwalb H, Schotz MC: Herapin-releasable and nonreleasable lipoprotein lipase in the perfused rat heart. J Biol Chem 256:10550–10554, 1981
Chajek T, Stein O, Stein Y. Lipoprotein lipase of cultured mesenchymal rat heart cells. I. Synthesis, secretion and releasability by heparin. Biochim Biophys Acta 528:456–465, 1978
Bagby GJ, Liu M-S, Spitzer JA: Lipoprotein lipase activity in rat heart myocytes. Life Sci 21:467–474, 1977
Vahouny GV, Tamboli A, Vander Maten M, Jansen H, Twu J-S, Schotz MC: Lipoprotein lipase activity of adult rat cardiocytes. Biochim Biophys Acta 620:63–69, 1980
Ramirez I, Kryski AJ, Ben-Zeev O, Schotz MC, Severson DL: Characterization of triacylglycerol hydrolase activities in isolated myocardial cells from rat heart. Biochem J 232:229–236, 1985
Chohan P, Cryer A: The lipoprotein lipase (clearing-factor lipase) activity of cells isolated from rat cardiac muscle. Biochem J 174:663–666, 1978
Chohan P, Cryer A. Lipoprotein lipase activity of rat cardiac muscle. Biochem J 186:873–879, 1980
Cryer A, Chohan P, Smith JM: Effectors of lipoprotein lipase secretion from isolated cardiac muscle cells incubated in vitro. Life Sci 29:923–929, 1981
Ramirez I, Severson DL: Effect of diabetes on acid and neutral triacylglycerol lipase and on lipoprotein lipase activities in isolated myocardial cells from rat heart. Biochem J 238:233–238, 1986
Severson DL, Carroll R, Kryski A, Ramirez I: Short-term incubation of cardiac myocytes with isoprenaline has no effect on heparin-releasable or cellular lipoprotein lipase activity. Biochem J 248:289–292, 1987
Kryski A, Kenno KA, Severson DL: Stimulation of lipolysis in rat heart myocytes by isoproterenol. Am J Physiol 248:H208-H216, 1985
Vannier C, Ailhaud GA: Continuous flow method for the study of lipoprotein lipase secretion in adipose cells. Biochim Biophys Acta 875:324–333, 1986
Semb H, Olivecrona T: Mechanisms for turnover of lipoprotein lipase in guinea pig adipocytes. Biochim Biophys Acta 921:104–115, 1987
Vannier C, Etienne J, Ailhaud G: Intracellular localization of lipoprotein lipase in adipose cells. Biochim Biophys Acta 875:344–354, 1986
Vannier C, Jansen H, Negrel R, Ailhaud G: Maturation and secretion of lipoprotein lipase in cultured adipose cells. J Biol Chem 260:4424–4431, 1985
Chajek-Saul T, Friedman G, Knobler H, Stein O, Etienne J, Stein Y. Importance of the different steps of glycosylation for the activity and secretion of lipoprotein lipase in rat preadipocytes studied with monensin and tunicamycin. Biochim Biophys Acta 837:123–134, 1985
Stewart JE, Schotz MC: Release of lipoprotein lipase activity from isolated fat cells. J Biol Chem 249:904–907, 1974
Vannier C, Amri E-Z, Etienne J, Negrel R, Ailhaud G: Study of lipoprotein lipase content in Ob17 preadipocytes during adipose conversion J Biol Chem 257:12387–12393, 1982
Cheng C-F, Oosta GM, Bensadoun A, Rosenberg RD: Binding of lipoprotein lipase to endothelial cells in culture. J Biol Chem 256:12893–12898, 1981
Shimada K, Gill PG, Silbert JE, Douglas WH, Fanburg BL: Involvement of cell surface heparin sulfate in the binding of lipoprotein lipase to cultured bovine endothelial cells. J Clin Invest 68:995–1002, 1981
Bengtsson G, Olivecrona T, Hook M, Riesenfeld J, Lindahl U: Interaction of lipoprotein lipase with native and modified heparin-like polysaccharides. Biochem J 189:625–633, 1980
Farmer BB, Mancina M, Williams ES, Watanabe AM: Isolation of calcium tolerant myocytes from adult rat hearts: review of the literature and description of a method. Life Sci 33:1–18, 1983
Frank JS, Brady AS, Farnsworth S, Mottino G: Ultrastructure and function of isolated myocytes after calcium depletion and repletion. Am J Physiol 250:H265-H275, 1986
Al-Jafari AA, Cryer A: The lipoprotein lipase of white adipose tissue. Biochem J 236:749–756, 1986
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Severson, D.L., Lee, M. & Carroll, R. Secretion of lipoprotein lipase from myocardial cells isolated from adult rat hearts. Mol Cell Biochem 79, 17–24 (1988). https://doi.org/10.1007/BF00229393
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00229393