Abstract
A cDNA clone of an S-locus glycoprotein gene (SLG) from Brassica oleracea specific to the S 29 self-incompatibility allele was used to detect homologous gene sequences in the S 63 haplotype. Two genomic fragments hybridised by this probe appeared to comprise an SLG and S-receptor kinase (SRK) gene pair, as previously described in B.oleracea. The complete DNA sequence of the putative receptor kinase gene was determined and found to predict a polypeptide structurally homologous to SRK proteins. Combined RFLP, stigma glycoprotein and pollination analysis of a segregating F2 family of plants indicated that the gene pair is linked to the S 63 incompatibility allele. Transcripts originating from the SRK-like gene were identified in stigma mRNA by DNA sequencing of amplified RT-PCR products but homologous transcripts were also found in leaf. An associated stigma-specific SLG transcript was not detected, suggesting that this SRK-like gene may have a function and tissue-specificity unrelated to SI and that the true SRK 63 allele may have a diverged DNA sequence.
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The DNA sequence data reported have been lodged in the EMBL, Genbank and DDBJ databases under the accession number Z18921.
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Oldknow, J., Trick, M. Genomic sequence of an SRK-like gene linked to the S-locus of a self-incompatible Brassica oleracea line. Sexual Plant Reprod 8, 247–253 (1995). https://doi.org/10.1007/BF00228945
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DOI: https://doi.org/10.1007/BF00228945