Skip to main content
Log in

A localized linkage map of the citrus tristeza virus resistance gene region

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

A localized genetic linkage map was developed of the region surrounding the citrus tristeza virus (CTV) resistance gene (designated Ctv) from Poncirus trifoliate L., a sexually compatible Citrus relative. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers in four intergeneric backcross families that were segregating for CTV resistance. Eight RAPD fragments were found that were consistently linked to Ctv in the four families. Map distances and locus order were determined with MAPMAKER 3.0, using the results obtained from 59 individuals in the largest family. Also, a consensus map was constructed with JOINMAP 1.3, using pooled results from the four backcross families. Marker orders were identical, except for 1 marker, on these independently developed maps. Family-specific resistance-associated markers were also identified, as were numerous susceptibility-associated markers. The identification of markers tightly linked to Ctv will enable citrus breeders to identify plants likely to be CTV-resistant by indirect, marker-assisted selection, rather than by labor-intensive direct challenge with the pathogen. These markers also provide a basis for future efforts to isolate Ctv for subsequent genetic manipulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bar-Joseph M, Marcus R, Lee RF (1989) The continuous challenge of citrus tristeza virus control. Annu Rev Phytopathol 27:291–316

    Google Scholar 

  • Barrett HC (1990) US 119, an intergeneric hybrid citrus scion breeding line. HortScience 25:1670–1671

    Google Scholar 

  • Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its relatives. Syst Bot 1:105–136

    Google Scholar 

  • Cai Q, Guy CL, Moore GA (1994) Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci. Theor Appl Genet 89:606–614

    Google Scholar 

  • Ding SQ, Zhang XN, Bao ZR, Liang MQ (1984) A new species of Poncirus from China (in Chinese). Acta Bot Yunnan 6:292–293

    Google Scholar 

  • Durham RE, Liou PC, Gmitter FG Jr, Moore GA (1992) Linkage of restriction fragment length polymorphisms and isozymes in Citrus. Theor Appl Genet 84:39–48

    Google Scholar 

  • Edwards MD, Helentjaris T, Wright S, Stuber CW (1992) Molecularmarker-facilitated investigation of quantitative traits loci in maize. Theor Appl Genet 83:765–774

    Google Scholar 

  • Fatmi A, Poneleit CG, Pfeiffer TW (1993) Variability of recombination frequencies in the Iowa Stiff Stalk Synthetic (Zea mays L.). Theor Appl Genet 86:859–866

    Google Scholar 

  • Garnsey SM, Lee RF (1988) Tristeza. In: Whiteside JO, Garnsey SM, Timmer LW (eds) Compendium of citrus diseases. APS Press, St. Paul, Minn., pp 48–50

    Google Scholar 

  • Garnsey SM, Barrett HC, Hutchison DJ (1981) Resistance to citrus tristeza virus in citrus hybrids as determined by enzyme-linked immunosorbent assay. Phytopathology 71:875 (abstr)

    Google Scholar 

  • Garnsey SM, Barrett HC, Hutchison DJ (1987) Identification of citrus tristeza virus resistance in citrus relatives and its potential applications. Phytophylactica 19:187–191

    Google Scholar 

  • Gmitter FG Jr, Grosser JW, Moore GA (1992) Citrus. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops. CAB Int, Wallingford, UK, pp 335–369

    Google Scholar 

  • Grill E, Somerville C (1991) Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet 226:484–490

    Google Scholar 

  • Gutierrez-E A, Moore GA, Jacono C, McCaffery M, Cline K (1992) Production of transgenic citrus plants expressing the citrus tristeza virus coat protein gene. Phytopathology 82:1148 (abstr)

    Google Scholar 

  • Haley SD, Miklas PN, Stavely JR, Byrum J, Kelly JD (1993) Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86:505–512

    Google Scholar 

  • Jarrell DC, Roose ML, Traugh SN, Kupper RS (1992) A genetic map of citrus based on segregation of isozymes and RFLPs in an intergeneric cross. Theor Appl Genet 84:49–56

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lee RF, Roistacher CN, Niblett CL, Lastra R, Rocha-Pena M, Garnsey SM, Yokomi RK, Gumpf DJ, Dodds JA (1992) Presence of Toxoptera citricidus in Central America: a threat to citrus in Florida and the United States. Citrus Ind 73:13–21

    Google Scholar 

  • Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0, 3rd edn. Whitehead Institute Technical Report, Cambridge, Mass.

  • Mansur LM, Orf J, Lark KG (1993) Determining the linkage of quantitative trait loci to RFLP markers using extreme phenotypes of recombinant inbreds of soybean (Glycine max L. Merr.). Theor Appl Genet 86:914–918

    Google Scholar 

  • Martin GB, Ganal MW, Tanksley SD (1992) Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci. Mol Gen Genet 223:25–32

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    CAS  PubMed  Google Scholar 

  • Miklas PN, Stavely JR, Kelly JD (1993) Identification and potential use of a molecular marker for rust resistance in common bean. Theor Appl Genet 85:745–749

    Google Scholar 

  • Moore GA, Gutierrez-E A, Jacono C, McCaffery M, Cline K (1993) Production of transgenic Citrus plants expressing the citrus tristeza virus coat protein gene. HortScience 28:512 (absrt)

    Google Scholar 

  • Niblett CL, Pappu HR, Pappu SS, Febres VJ, Manjunath KL, Lee RF, Grosser JW, Schell J (1993) Progress on the characterization and control of citrus tristeza virus. Proc Fla State Hortic Soc 106:99–102

    Google Scholar 

  • Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    CAS  Google Scholar 

  • Penner GA, Chong J, Levesque-Lemay M, Molnar SJ, Fedak G (1993) Identification of a RAPD marker linked to the oat stem rust gene Pg3. Theor Appl Genet 85:702–705

    Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Tanksley SD, Medina-Filho H, Rick CM (1982) Use of naturally occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backcross of tomato. Heredity 49:11–25

    Google Scholar 

  • Torres AM, Mau-Lastovika T, Williams TE, Soost RK (1985) Segregation distortion and linkage of Citrus and Poncirus isozyme genes. J Hered 76:289–294

    Google Scholar 

  • Weeden NF, Timmerman GM, Hemmat M, Kneen BE, Lodhi MA (1992) Inheritance and reliability of RAPD markers. In: Proc Sym Applic RAPD Technol Plant Breed. Minneapolis, Minn., Crop Science Society of America, pp 12–17

    Google Scholar 

  • Weisemann JM, Matthews BF, Devine TE (1992) Molecular markers located proximal to the soybean cyst nematode resistance gene, Rhg4. Theor Appl Genet 85:136–138

    Google Scholar 

  • Yokomi RK (1993) The brown citrus aphid: its role in the transmission of CTV. In: Proc Citrus Tristeza Virus Brown Citrus Aphid Workshop. Florida Citrus Mutual, pp 19–20

  • Yoshida T (1985) Inheritance of susceptibility to citrus tristeza virus in trifoliate orange, (in Japanese with English summary). Bull Fruit Tree Res Stn Ser B (Okitsu) 12:17–26

    Google Scholar 

  • Yoshida T (1993) Inheritance of immunity to citrus tristeza virus of trifoliate orange in some citrus intergeneric hybrids (in Japanese with English summary). Bull Fruit Tree Res Stn 25:33–43

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Salamini

Florida Agricultural Experimental Station Journal Series No. R-04491

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gmitter, F.G., Xiao, S.Y., Huang, S. et al. A localized linkage map of the citrus tristeza virus resistance gene region. Theoret. Appl. Genetics 92, 688–695 (1996). https://doi.org/10.1007/BF00226090

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226090

Key words

Navigation