Skip to main content
SpringerLink
Log in

Effect of the phenylurea cytokinin 4-PU-30 on the growth and protein composition of maize seedlings

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The action of the phenylurea cytokinin 4-PU-30 on maize seedling growth, photosynthetic parameters, and leaf protein composition was investigated. The applied phenylurea cytokinin increased leaf growth and photosynthetic activity of the seedlings. It also elevated chlorophyll and total nitrogen content in leaves, as well as the quantity of individual protein fractions. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) of soluble proteins (albumins plus globulins) revealed four protein polypeptides with molecular masses of 27,000, 24,000, 17,000, and 15,000. Comparison of the polypeptides from treated plants with relevant polypeptides from control plants showed some significant, quantitative differences. New proteins, of similar molecular mass, may be produced in treated plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alexieva VS, Karanov EN (1992) Effect of N 1-(2-chloro-4-pyridiyl)-N 2-phenylurea (4-PU-30) on wheat yield. In: Kaminek M, Mok DW (eds) Physiology and biochemistry of cytokinins in plants. Academia Publishing bv, The Hague, The Netherlands, pp 249–251

    Google Scholar 

  • Ananiev E, Karagyozov L, Karanov E (1987) Effect of cytokinins on ribosomal RNA gene expression in excised cotyledons of Cucurbita pepo. Planta 170:370–378

    Article  CAS  Google Scholar 

  • Arnon D (1949) Copper enzymes in isolated chloroplast phenol oxydase in Beta vulgaris. Plant Physiol 4:1–15

    Google Scholar 

  • De Jong WW, Zweers A, Cohen LH (1978) Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochem Biophys Res Commun 82:532–539

    Article  PubMed  Google Scholar 

  • Dimitrova A, Vassileva V (1987) Action of some growth regulators on the RuDP-carboxylase, RuDP-oxygenase ratio in maize leaves. In: Proc of the Inter Youth Symp Plant Metabolism Regulation, Sofia 4:147–151

    Google Scholar 

  • Devlin R, Zbifc I, Nowicka S (1989) The effect of thidiazuron on some plant growth systems. In: Proc 16 annual meeting Plant Growth Regul Soc America, 16:99–103

    Google Scholar 

  • Feller U, Soong T, Hageman R (1977) Leaf proteolytic activities and senescence during grain development of field-grown corn (Zea mays L). Plant Physiol 59:290–294

    PubMed  CAS  Google Scholar 

  • Hayes PM, Patrick JW (1985) Photosynthetic transport in stems of Phaseolus vulgaris L treated with gibberellic acid, indole-3-acetic acid or kinetin. Effects at the site of hormone application. Planta 166:371–375

    Article  CAS  Google Scholar 

  • Hodgson RH, Snyder RH (1988) Thidiazuron effects on Malvacea corn Zea mays and soybean Glicine max. Weed Technol 2:342–349

    CAS  Google Scholar 

  • Huffaker RC, Peterson LW (1974) Protein turnover in plants and possible means of its regulator. Ann Rev Plant Physiol 25:363–368

    Article  CAS  Google Scholar 

  • Karanov E, Iliev L, Georgiev G, Tsolova M, Alexieva V, Puneva I (1992) Physiology and application of phenylurea cytokinins In: Karssen CM, Van Loon LC, Vreugdenhil P (ed) Progress in plant growth regulation. The Netherlands, pp 842–851

  • Klyachko N, Schramm I, Kulaeva O (1987) Effect of thidiazuron and cartolin on polysome formation and growth of pumpkin cotyledons. Fiziol Rast (Moskow) 34:319–323

    CAS  Google Scholar 

  • Kulaeva O, Baskanov Y, Borisova N, Kuznetsov V, Tsibulya L, Shapovalov A (1992) Investigation of cytokinin properties of the defoliant DROPP and the herbicide DPX-4189. Fiziol Rast (Moskow) 29:266–273

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Landry J, Moureaux T (1970) Hétérogénéite des glutelines du grain de mais: Extraction sélective et composition en acides amines des trois fractions isolées. Bull Soc Chem Biol 52:1021–1037

    CAS  Google Scholar 

  • Landry J, Paulis JW, Wall JS (1987) Chromatographic and electrophoretic analysis of zein heterogeneity. J Cereal Sci 5:51–60

    Article  CAS  Google Scholar 

  • Letham PS (1978) Cytokinins In: Letham PS, Goodwin PB, Higgins TY (eds) Phytohormones and related compounds: A comprehensive treatise, vol 1. Elsevier North-Holland Biochemical Press, Amsterdam Oxford New York, pp 205–251

    Google Scholar 

  • MacKinney G (1941) Absorption of light by chlorophyll solution. J Biol Chem 140:315–322

    CAS  Google Scholar 

  • Martin C, Thimann KV (1972) The role of protein synthesis in the senescence of leaves. I. The formation of protease. Plant Physiol 49:64–68

    PubMed  CAS  Google Scholar 

  • Martin M, Sabater B (1989) Transnational control of chloroplast protein synthesis during senescence of barley leaves. Physiol Plantarum 75:374–381

    Article  CAS  Google Scholar 

  • Mok M, Mok D, Armstrong D, Shudo K, Isogai J, Okamoto T (1982) Cytokinin activity of N-phenyl-N′-1,2,3,thidiazol-5-yl urea (thidiazuron). Phytochemistry 21:1509–1511

    CAS  Google Scholar 

  • Mok M, Mok D, Turner J, Mujer C (1987) Biological and biochemical effects of cytokinin active phenylurea derivatives in tissue culture system. Hort Sci 22:1194–1196

    CAS  Google Scholar 

  • Nickell LG (1986) Effects of N-(2-chloro-4-pyridyl)-N′-phenylurea on grapes and other crops. In: Proceedings of 13th Annual Meeting of the Plant Growth Regulator Society of America, pp 236–241

  • Nickell LG (1991) Use of growth regulating chemical. In: Raghavendra AS (ed) Physiology of trees. John Wiley & Sons, New York, pp 467–487

    Google Scholar 

  • Ohya T, Suzuki H (1990) Benzyladenine and light-stimulated plastid protein synthesis in excised cucumber cotyledons. Plant Physiol Biochem (Paris) 28:27–35

    CAS  Google Scholar 

  • Okamoto T, Shudo K, Takahashi T, Yatsunami T, Isogai Y, Yamada K (1978) Shoot formation of tobacco callus by various cytokinin active ureas and pyrimidines. Chem Pharm Bull 26:3250–3252

    CAS  Google Scholar 

  • Okamoto T, Shudo K, Takahashi T, Kawachi S, Isogai J (1981) 4-Pyridylureas are surprisingly potent cytokinins. The structure-activity relationship. Chem Pharm Bull 29:3748–3750

    CAS  Google Scholar 

  • Raschke K (1975) Stomatal action. Ann Rev Plant Physiol 26:309–340

    Article  CAS  Google Scholar 

  • Romanov GA (1990) Cytokinins and tRNAs: A new insight into the old problem. Fiziol Rast (Moskow) 37:1196–1210

    Google Scholar 

  • Takahashi S, Shudo K, Okamoto T, Yamato K, Isogai Y (1978) Cytokinin activity of N-phenyl-N′-(4-pyridyl) urea derivatives. Phytochemistry 17:1201–1207

    Article  CAS  Google Scholar 

  • Thomas TH (1985) Hormonal control of assimilate movement and compartmentation. In: Bipp M (ed) Plant growth substances. Springer-Verlag, Berlin, pp 350–359

    Google Scholar 

  • Thomas TH, Blakesly D (1987) Cytokinins. Plant hormones in search of a role. Sittingbourne

  • Tsenova E, Vassileva V (1990) Physiological effects of synthetic growth regulator PP thidiazuron (Dropp) in higher plants. In: Proc X Nat Conf Fiziol na Rast. Sofia 8:250–256

    Google Scholar 

  • Weber K, Osborn M (1969) The reliability of molecular weight determination by dodecyl sulfate-polyarylamide gel electrophoresis. J Biol Chem 244:4406–4412

    PubMed  CAS  Google Scholar 

  • Wilson CM (1979) Studies and critique of amido black 10 B, Coomassie Blue R and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis. Anal Biochem 96:263–278

    Article  PubMed  CAS  Google Scholar 

  • Wilson CM (1981) Variations in soluble endosperm proteins of corn (Zea mays L). Inbreds as detected by disc electrophoresis. Cereal Chem 58:401–408

    CAS  Google Scholar 

  • Wilson CM (1986) Serial analysis of zein by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis. Plant Physiol 82:196–202

    PubMed  CAS  Google Scholar 

  • Yordanov I, Popov K, Chickev P (1969) Use of 14C in the study intensity and products of photosynthesis. In: Proc I Nat Conf on the use of ionising radiation and isotopes in biology and agriculture, Sofia, pp 59–72

Download references

Author information

Authors and Affiliations

  1. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg. 21, 1113, Sofia, Bulgaria

    B. J. Stefanov, L. K. Iliev & N. I. Popova

Authors
  1. B. J. Stefanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. K. Iliev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. I. Popova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stefanov, B.J., Iliev, L.K. & Popova, N.I. Effect of the phenylurea cytokinin 4-PU-30 on the growth and protein composition of maize seedlings. J Plant Growth Regul 13, 231–234 (1994). https://doi.org/10.1007/BF00226042

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226042

Keywords

Search

Navigation