Skip to main content
SpringerLink
Log in

Protective effect of exogenous polyamines against atrazine in pea plants

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effect of exogenously applied polyamines in reversing the effect of atrazine stress on pea seedlings (Pisum sativum L., cv. Koray) was investigated. The plants treated with combinations of atrazine (14 mM) + spermidine (1 mM) and atrazine (14 mM) + spermine (1 mM) possessed improved growth (30–35% increase of leaf area and 10–20% increase of fresh weight 10 days after treatment) and chlorophyll content (50–60% increase) in comparison with atrazine-(14 mM) treated plants. Spermine and spermidine also diminished the inhibitory effect of atrazine on gas exchange and photosystem II function. This fact supports the hypothesis of Yordanov and Goltsev (1990, Plant Physiol 4:42–51) that the interaction of polyamines with the thylakoid membrane surface led to their stacking, to separation of the photosystems, and to the association of light-harvesting complex II with the photosystem II core complex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altman A (1982a) Retardation of radish leaf senescence by polyamines. Physiol Plant 54:189–193

    Article  CAS  Google Scholar 

  • Altman A (1982b) Polyamines and wounded storage tissues. Inhibition of RNase activity and solute leakage. Physiol Plant 54:194–198

    Article  CAS  Google Scholar 

  • Altman A, Friedman R, Amir D, Levin N (1982) Polyamine effects and metabolism in plants under stress conditions. In: Wareing P (ed) Plant growth substances. Academic Press, New York, pp 483–494

    Google Scholar 

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15

    PubMed  CAS  Google Scholar 

  • Bagni N (1986) The function and metabolism of polyamines in plants. Acta Hortic Growth Regul 179:95–103

    Google Scholar 

  • Barbiroli B, Mezzetti G, Moruzzi M (1989) Polyamines and initiation of protein synthesis in eukaryotes. In: Bachrach U, Heimer Y (eds) The physiology of polyamines. CRC Press, Boca Raton, pp 125–132

    Google Scholar 

  • Besford R, Richardson C, Campos J, Tiburcio A (1993) Effect of polyamines on stabilisation of molecular complexes in thylakoid membranes of osmotically stressed oat leaves. Planta 189:201–206

    Article  CAS  Google Scholar 

  • Bors W, Langebartels C, Michel C, Sandermann H (1989) Polyamine as radical scavengers and protectants against ozone damage. Phytochemistry 28:1589–1595

    Article  CAS  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Brenchley R, Appleby A (1971) Effect of magnesium and photoperiod on atrazine toxicity to tomatoes. Weed Sci 19:524–525

    CAS  Google Scholar 

  • Cohen A, Zalik S (1978) Magnesium replacement by polyamines in higher plant cell-free polyphenylalanine synthesis. Phytochemistry 17:113–118

    Article  CAS  Google Scholar 

  • Ebert E, Dumford S (1976) Effects of triazine herbicides on the physiology of plants. Residue Rev 65:1–103

    Google Scholar 

  • Flores H (1991) Changes in polyamine metabolism in response to abiotic stress. In: Slocum RD, Flores HE (eds) Biochemistry and physiology of polyamines in plants. CRC Press, Boca Raton, pp 213–228

    Google Scholar 

  • Flores H, Filner P (1985) Polyamine catabolism in higher plants: Characterisation of pyrroline dehydrogenase. J Plant Growth Regul 3:277–291

    Article  CAS  Google Scholar 

  • Fuerst E, Norman M (1991) Interactions of herbicides with photosynthetic electron transport. Weed Sci 39:458–464

    CAS  Google Scholar 

  • Fuhrer J, Kaur-Sawhney R, Shih L, Galston A (1982) Effects of exogenous 1,3-diaminopropan and spermidine on senescence of oat leaves. Plant Physiol 70:1597–1600

    Article  PubMed  CAS  Google Scholar 

  • Galston AW, Kaur-Sawhney R (1990) Polyamines in plant physiology. Plant Physiol 94:406–410

    PubMed  CAS  Google Scholar 

  • Holt J, Radoshevich S, Stemler A (1983) Differential efficiency of photosynthetic oxygen evolution in flashing light in triazine-susceptible biotypes of Senecio vulgaris L. Biochim Biophys Acta 722:245–255

    Article  CAS  Google Scholar 

  • Iliev L (1991) Symmetric triazines—Metabolism, growth regulating and herbicide activity (mechanism of action). Plant Physiol (Sofia) 1:31–40

    Google Scholar 

  • Isola M, Franzoni L (1989) Inhibition of net synthesis of ribonuclease by polyamines in potato tuber slices. Plant Sci 63:39–45

    Article  CAS  Google Scholar 

  • Kaur-Sawhney R, Galston A (1979) Interaction of polyamines and light on biochemical processes involved in leaf senescence. Plant Cell Environ 2:189–196

    Article  Google Scholar 

  • Kaur-Sawhney R, Shih L, Galston A (1982) Relation of polyamine biosynthesis to the initiation of sprouting in potato tubers. Plant Physiol 69:411–415

    PubMed  CAS  Google Scholar 

  • Krause G, Weis E (1991) Chlorophyll fluorescence and photosynthesis: The basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349

    Article  CAS  Google Scholar 

  • Langebartels C, Kerner K, Leonardi S, Schraudner M, Trost M, Heller W, Sandermann H (1991) Biochemical plant responses to ozone. I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol 95:882–889

    PubMed  CAS  Google Scholar 

  • Lichtenthaler H (1988) In vivo chlorophyll fluorescence as a tool for stress detection in plants. In: Lihtenthaler H (ed) Applications of chlorophyll fluorescence. Kluwer Academic Publishers, pp 129–142

  • Nivison H, Stocking C (1983) Ribulose bisphosphate carboxylase synthesis in barley leaves. Plant Physiol 73:906–911

    PubMed  CAS  Google Scholar 

  • Percival M, Webber A, Baker N (1984) Evidence for the role of the LHC a/b protein complex on PS II heterogeneity. Biochem Biophys Acta 767:582–589

    Article  CAS  Google Scholar 

  • Popovic R, Kyle D, Cohen A, Zalic S (1979) Stabilization of thylakoid membranes by spermine during stress induced senescence of barley leaf discs. Plant Physiol 64:721–726

    PubMed  CAS  Google Scholar 

  • Preston C, Holtum A, Powels S (1992) Do polyamines contribute to paraquat resistance in Hordeum vulgare? Photosynth Res 34:193 (abstract)

    Google Scholar 

  • Renger G, Fromme R, Hagemann R (1988) The modification of atrazine by the redox state of the endogenous high-spin iron and specific proteolytic enzymes in photosystem II membrane fragments and intact thylakoids. Biochem Biophys Acta 935:173–183

    Article  CAS  Google Scholar 

  • Rugolo M, Antognoni F, Flamigni A, Zannoni D (1991) Effects of polyamines on the oxidation of exogenous NADH by Jerusalem artichoke (Helianthus tuberosus) mitochondria. Plant Physiol 95:157–163

    PubMed  CAS  Google Scholar 

  • Shih L, Kaur-Sawhney R, Fuhrer J, Samanta S, Galston A (1982) Effects of exogenous 1,3-diaminopropane and spermidine on senescence of oat leaves. Inhibition of RNase activity, ethylene production and chlorophyll loss as related to polyamine content. Plant Physiol 70:194–198

    Google Scholar 

  • Srivastava S, Smith T (1982) The effect of some oligoamines and guanidines on membrane permeability in higher plants. Phytochemistry 21:997–1008

    Article  CAS  Google Scholar 

  • Tsonev T, Sergiev I (1993) Leaf area measurement using hand scanner. Photosynthetica 29:625–630

    Google Scholar 

  • Tyystjarvi E, Karunen J (1990) A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients. Photos Res 26:127–132

    Article  Google Scholar 

  • Yordanov I, Goltsev V (1990) The protective effect of some polyamines on thylakoid membrane functioning. Plant Physiol (Sofia) 4:42–51

    Google Scholar 

  • Zheleva D, Alexieva V, Karanov E (1993a) Influence of atrazine on free and bound polyamine levels in pea leaves. J Plant Physiol 141:281–285

    CAS  Google Scholar 

  • Zheleva D, Alexieva V, Karanov E (1993b) Influence of atrazine on polyamine biosynthetic enzymes. Comp Rend Acad Bul Sci 10 (in press)

Download references

Author information

Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113, Sofia, Bulgaria

    D. Zheleva, T. Tsonev, I. Sergiev & E. Karanov

Authors
  1. D. Zheleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Tsonev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Sergiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Karanov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zheleva, D., Tsonev, T., Sergiev, I. et al. Protective effect of exogenous polyamines against atrazine in pea plants. J Plant Growth Regul 13, 203–211 (1994). https://doi.org/10.1007/BF00226038

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226038

Keywords

Search

Navigation