Skip to main content
SpringerLink
Log in

A RFLP linkage map of Sorghum bicolor (L.) Moench

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

A RFLP linkage map of sorghum composed principally of markers detected with sorghum low-copy-number nuclear DNA clones has been constructed. The map spans 1789 cMs and consists of 190 loci grouped into 14 linkage groups. The 10 largest linkage groups consist of from 10 to 24 markers and from 103 to 237 cMs, and the other 4 linkage groups consist of from 2 to 5 markers and from 7 to 62 cMs. The map was derived in Sorghum bicolor ssp. bicolor by analysis of a F2 population composed of 50 plants derived from a cross of IS 3620C, a guinea line, and BTx 623, an agronomically important inbred line derived from a cross between a zera zera (a caudatum-like sorghum) and an established kafir line. The restriction fragment length polymorphism (RFLP) frequency detected in this population using polymerase chain reaction (PCR)-amplifiable low-copy-number sorghum clones and five restriction enzymes was 51%. A minimal estimate of the number of clones that detect duplicate sequences is 11 %. Null alleles occurred at 13% of the mapped RFLP loci.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baxter-Gabbard KL (1972) A simple method for the large-scale preparation of sucrose gradients. FEBS Lett 20:117–119

    Google Scholar 

  • Beavis WD, Grant B (1991) A linkage map based on information from four F2 populations of maize (Zea mays L.). Theor Appl Genet 82:636–644

    Google Scholar 

  • Berhan AM, Hulbert SH, Bulter LG, Bennetzen JL (1993) Structure and evolution of the genomes of Sorghum bicolor and Zea mays. Theor Appl Genet 86:598–604

    Google Scholar 

  • Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Coe EH Jr, Hoisington DA, Neuffer MG (1990) Linkage map of corn (Zea mays L.). In: O'Brien SJ (ed) Genetic maps. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp 6.39–6.67

    Google Scholar 

  • Davis PB, Pearson CK (1978) Characterization of density gradients prepared by freezing and thawing a sucrose solution. Anal Biochem 91:343–349

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75

    Google Scholar 

  • Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72:761–769

    Google Scholar 

  • Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447

    Google Scholar 

  • Hulbert SH, Richter TE, Axtell JD, Bennetzen JL (1990) Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes. Proc Natl Acad Sci USA 87:4251–4255

    Google Scholar 

  • Keim P, Diers BW, Shoemaker RC (1990) Genetic analysis of soybean hard seededness with molecular markers. Theor Appl Genet 79:465–469

    Google Scholar 

  • Kesseli RV, Paran I, Michelmore RW (1990) Genetic linkage map of lettuce (Lactuca sativa, 2n = 18). In: O'Brien SJ (ed) Genetic maps. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 6.100–6.102

    Google Scholar 

  • Klein-Lankhorst R, Rietveld P, Machiels B, Verkerk R, Weide R, Gebhardt D, Koornneef M, Zabel P (1991) RFLP markers linked to the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 81:661–667

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Google Scholar 

  • Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln S (1991) A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34:543–552

    Google Scholar 

  • Maniatis F, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829

    Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325

    Google Scholar 

  • Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with resistance in tomato. Crop Sci 27:797–803

    Google Scholar 

  • Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated linkage map of common bean 2. Development of an RFLP-based linkage map. Theor Appl Genet 85:513–520

    Google Scholar 

  • Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73:350–356

    Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Google Scholar 

  • Paterson AH, DeVerna JW, Lanini B, Tanksley SD (1990) Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics 124:735–742

    Google Scholar 

  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197

    Google Scholar 

  • Reed KC, Mann DA (1985) Rapid transfer of DNA agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Google Scholar 

  • Whitkus R, Doebley J, Lee M (1992) Comparative genome mapping of sorghum and maize. Genetics 132:1119–1130

    Google Scholar 

  • Xu GW, Magill CW, Hart GE (1993) Primers that amplify inserts in a multi-cloning site also hybridize to Sorghum bicolor (L.) Moench DNA. Genome 36:198–201

    Google Scholar 

  • Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm2a gene in tomato. Genetics 120:579–585

    Google Scholar 

  • Yu ZH, Mackill DJ, Bonman JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A&M University, 77843, College Station, TX, USA

    G. -W. Xu & G. E. Hart

  2. Department of Plant Pathology and Microbiology, Texas A&M University, 77843, College Station, TX, USA

    C. W. Magill

  3. Department of Soil and Crop Sciences, USDA-ARS in Cooperation with Texas A&M University, 77843, College Station, TX, USA

    K. F. Schertz

Authors
  1. G. -W. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. W. Magill
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. F. Schertz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. E. Hart
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J.W. Snape

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, G.W., Magill, C.W., Schertz, K.F. et al. A RFLP linkage map of Sorghum bicolor (L.) Moench. Theoret. Appl. Genetics 89, 139–145 (1994). https://doi.org/10.1007/BF00225133

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00225133

Key words

Search

Navigation