Summary
Changes in phosphorylation of ribosomal protein S6 during heat shock, induction of thermotolerance and recovery from heat shock at different stages of Blastocladiella emersonii development were investigated. Independently of the initial state of S6 phosphorylation (maximal or intermediate), a rapid and complete dephosphorylation of S6 is induced by heat shock and S6 remains unphosphorylated during the acquired thermotolerance. During recovery from heat shock rephosphorylation of S6 occurs always to the levels characteristic of that particular stage, coincidently with the turn off of heat shock protein synthesis.
Similar content being viewed by others
References
Traugh JA: Regulation of protein synthesis by phosphorylation. In: Litwack G (ed) Biochemical Actions of Hormones, vol 8. Academic Press, New York, 1981, pp 167–208
Leader DP: The control of phosphorylation of ribosomal proteins. In: Cohen P (ed) Molecular Aspects of Cellular Regulation, vol. 1. Elsevier, North Holland, Amsterdam, 1980, pp 203–233
Thomas G, Martin-Perez J, Siegmann M, Otto AM: The effect of serum, EGF, PGF2α and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis. Cell 30:235–242, 1982
Trevillyan JM, Perisic O, Traugh JA, Byus CV: Insulin- and phorbol ester-stimulated phosphorylation of ribosomal protein S6. J Biol Chem 260:3041–3044, 1985
Blenis J, Spivack JG, Erickson RL: Phorbol ester, serum, and Rous sarcoma virus transforming gene product induce similar phosphorylation of ribosomal protein S6. Proc Natl Acad Sci USA 81:6408–6412, 1984
Maller JL, Foulkes JG, Erickson E, Baltimore D: Phosphorylation of ribosomal protein S6 on serine after microinjection of the Abelson murine leukemia virus tyrosine-specific protein kinase into Xenopus oocytes. Proc Natl Acad Sci USA 82:272–276, 1985
Ballinger DG, Hunt T: Fertilization of sea urchin eggs is accompanied by 40S ribosomal subunit phosphorylation. Dev Biol 87:277–285, 1981
Duncan R, McConkey EH: Preferential utilization of phosphorylated 40-S ribosomal subunits during initiation complex formation. Eur J Biochem 123:535–538, 1982
Burkhard SJ, Traugh JA: Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation. J Biol Chem 258:14003–14008, 1983
Lindquist S: The heat-shock response. Annu Rev Biochem 55:1151–1191, 1986
Scott MP, Pardue ML: Translational control in lysates of Drosophila melanogaster cells. Proc Natl Acad Sci USA 78:3353–3357, 1981
Glover CVC: Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila. Proc Natl Acad Sci USA 79:1781–1785, 1982
Scharf KD, Never L: Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cultures. Cell 30:427–437, 1982
Kennedy IM, Burden RH, Leader DP: Heat shock causes diverse changes in the phosphorylation of the ribosomal proteins of mammalian cells. FEBS Lett 169:267–273, 1984
Olsen AS, Triemer DF, Sanders MM: Dephosphorylation of S6 and expression of the heat shock response in Drosophila melanogaster Mol Cell Biol 3:2017–2027, 1983
Bonato MCM, Silva AM, Maia JCC, Juliani MH: Phosphorylation of ribosomal protein S6 in the aquatic fungus Blastocladiella emersonii. Eur J Biochem 144:597–606, 1984
Bonato MCM, Silva AM, Gomes SL, Maia JCC, Juliani MH: Differential expression of heat-shock proteins and spontaneous synthesis of HSP70 during the life cycle of Blastocladiella emersonii. Eur J Biochem 163:211–220, 1987
Silva AM, Maia JCC, Juliani MH: Developmental changes in translatable RNA species and protein synthesis during sporulation in the aquatic fungus Blastocladiella emersonii. Cell Differ 18:263–274, 1986
O'Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021, 1975
Desmarquets G, Cortay JC, Cozzonne AJ: Two-dimensional analysis of proteins phosphorylated in E. coli cells. FEBS Lett 173:337–341, 1984
Lastick SM, McConkey EH: Exchange and stability of HeLa ribosomal proteins in vivo. J Biol Chem 251:2867–2876, 1976
Bonato MCM, Maia JCC, Juliani MH: Analysis of Blastocladiella emersonii ribosomal proteins in four twodimensional gel electrophoresis systems. Brazilian J Med Biol Res 18:143–150, 1985
Gressner AM, Wool IG: The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated. J Biol Chem 249, 6917–6925, 1974
Li GC, Werb Z: Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts. Proc Natl Acad Sci USA 79, 3218–3222, 1982
Silva AM, Juliani MH, Araujo JJC, Bonato MCM: Acquisition of thermotolerance during development of Blastocladiella emersonii. Biochem Biophys Res Commun, 144:491–498, 1987
Silva AM, Maia JCC, Juliani MH: Changes in the pattern of protein synthesis during zoospore germination in Blastocladiella emersonii. J Bacteriol 169:2069–2078, 1987
Kruppa J, Clemens MJ: Differential kinetics of changes in the state of phosphorylation of ribosomal protein S6 and in the rate of protein synthesis in MPCII cells during tonicity shifts. EMBO J 3:95–100, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
da Silva, A.M., Juliani, M.H. & Bonato, M.C.M. Effect of heat shock on S6 phosphorylation during the development of Blastocladiella emersonii . Mol Cell Biochem 78, 27–35 (1987). https://doi.org/10.1007/BF00224421
Issue Date:
DOI: https://doi.org/10.1007/BF00224421