Summary
Previous immunohistochemical data have shown that the 44-kDal bone phosphoprotein (44K BPP, also called sialoprotein I or oestopontin) recently isolated in our laboratory was synthesized by osteoblasts and osteocytes and was expressed early during differentiation of boneforming cells. We report here the presence of 44K BPP antigenicity at certain ectopic sites, namely, the proximal-convoluted tubule of the kidney, neurons, sensory and secretory cells in the internal ear. To insure specificity and reproducibility, different immunohistochemical methods were used and affinity-purified antibodies against two separate preparations of pure 44K BPP were tested. In the cells of the proximal-convoluted tubule, 44K BPP immunoreactivity was observed within apical endocytotic vacuoles and within lysosomes. This staining thus correlates with the degradation of the 44K BPP epitope which we previously demonstrated to occur in serum. On the other hand, in the neurons of the acoustic ganglion and the sensory cells of the macula, 44K BPP immunoreactivity was associated with the Golgi apparatus indicating synthesis and secretion by these cells. The finding that the 44K BPP (or a structurally related molecule) is synthesized by neurons and neuroepithelial cells deserves further investigation with respect to a possible embryologie relationship between neuroectodermal cells and the precursors of some bone forming-cells of the skull.
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References
Bariety J, Druet P, Laliberte F, Sapin C, Belair MF, Paing M (1978) Ultrastructural evidence, by immunoperoxidase technique, for a tubular reabsorption of endogenous albumin in normal rat. Lab Invest 38:175–180
Batten EH (1958) The origin of the acoustic ganglion of the sheep. J Embryol Exp Morphol 6:597–615
Beckstead JH (1985) Optimal antigen localization on human tissues using aldehyde-fixed plastic-embedded sections. J Histochem Cytochem 33:954–958
Burstone MS (1961) Histochemical demonstration of phosphatases in frozen sections with naphthol AS-phosphates. J Histochem Cytochem 9:146–153
Butler WT (1984) Matrix macromolecules of bone and dentin. Coll Rel Res 4:297–307
Carone FA, Peterson DR, Oparil S, Pullman TN (1979) Renal tubular transport and catabolism of proteins and peptides. Kidney Int 16:271–278
Cohen-Solal L, Lian JB, Kossiva D, Glimcher MJ (1979) Identification of organic phosphorus covalently bound to collagen and non-collagenous proteins of chicken bone matrix. Biochem J 177:81–98
Davidoss MS (1986) Immunocytochemistry-possibilities for detection of different tissue antigens and establishment of the functional role of cells. Acta Histochem [Suppl] 33:175–193
Finkelman RD, Butler WT (1985) Appearance of dentin γ-carboxyglutamic acid-containing proteins in developing rat molars in vitro. J Dent Res 64:1008–1015
Fisher LW, Termine J (1985) Noncollagenous proteins influencing the local mechanisms of calcification. Clin Orthop 200:362–385
Franzén A, Heinegård D (1985) Proteoglycans and proteins of rat bone: purification and biosynthesis of major noncollagenous macromolecules. In: Butler WT (ed) The chemistry and biology of mineralized tissues. EBSCO, Media, Birmingham, pp 132–141
Galaske RG, Van Liew JB, Feld LG (1979) Filtration and reabsorbtion of low-molecular-weight protein in the rat kidney. Kidney Int 16:394–403
Graham RC, Karnowsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Graham RC, Kellermeyer RW (1968) Bovine lactoperoxidase as a cytochemical protein tracer for electron microscopy. J Histochem Cytochem 16:275–278
Hanker JS, Yates PE, Metz CB, Rustioni A (1977) A new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. Histochem J 9:789–792
Horster M, Larsson L (1976) Mechanism of fluid absorption during proximal tubule development. Kidney Int 10:348–363
Karnowsky MJ, Rice DF (1969) Exogenous cytochrome c as an ultrastructural tracer. J Histochem Cytochem 17:751–753
Kimura R, Lundquist PG, Wersäll J (1964) Secretory epithelial linings in the ampullae of the guinea pig labyrinth. Acta Otolaryngol (Stockh) 57:517–530
Knowlton VY (1967) Correlation of the development of membranous and bony labyrinths, acoustic ganglia, nerves and brain centers of the chick embryo. J Morphol 121:179–208
Laferte S, Krantz MJ (1983) Purification and characterization of a 50,000 mol wt glycoprotein antigen and localization of determinants involved in cross-reactivity with carcinoembryonic antigen. Mol Immunol 20:421–431
Lane D, Koprowsky H (1982) Molecular recognition and the future of monoclonal antibodies. Nature 296:200–202
Lee SL, Glimcher MJ (1981) Purification, composition and P31 NMR spectroscopic properties of a non-collagenous phosphoprotein isolated from chicken bone matrix. Calcif Tissue Int 33:385–394
Le Lievre CS (1974) Role de cellules mesectodermiques issues des cretes neurales cephaliques dans la formation des arcs branchiaux et du squelette viceral. J Embryol Exp Morphol 31:453–477
Le Lievre CS (1978) Participation of neural crest-derived cells in the genesis of the skull in birds. J Embryol Exp Morphol 47:17–37
Maack T, Johnson V, Kau ST, Figueiredo J, Sigulem D (1979) Renal filtration, transport and metabolism of low-molecularweight proteins: a review. Kidney Int 16:251–270
Mark MP, Prince CW, Gay S, Austin RL, Bhown M, Finkelman RD, Butler WT (1987a) A comparative immunocytochemical study on the subcellular distributions of a 44 kDal bone phosphoprotein and bone γ-carboxyglutamic acid (Gla)-containing protein in osteoblasts. J Bone Mineral Res 2:337–346
Mark MP, Prince CW, Oosawa T, Gay S, Bronckers ALJJ, Butler WT (1987b) Immunohistochemical demonstration of a 44 kDal phosphoprotein in developing rat bones. J Histochem Cytochem 35:707–715
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Nakai Y, Hilding D (1968) Vestibular endolymph-producing epithelium. Acta Otolaryngol (Stockh) 66:120–128
Narayanan CH, Narayanan Y (1978) Determination of the embryonic origin of the mesencephalic nucleus of the trigeminal nerve in birds. J Embryol Exp Morphol 43:85–105
Oldberg A, Franzén A, Heinegård D (1986) Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell binding sequence. Proc Natl Acad Sci USA 83:8819–8823
Pearson AA (1949) Further observations on the mesencephalic root of the trigeminal nerve. J Comp Neurol 91:147–195
Prince CW, Oosawa T, Butler WT, Tomana M, Bhown AS, Bhown M, Schrohenloher RE (1987) Isolation, characterization and biosynthesis of a phosphorylated protein from rat bone. J Biol Chem 262:2900–2907
Stauss W (1964) Cytochemical observations on the relationship between lysosomes and phagosomes in kidney and liver by combined stainings for acid phosphatase and intravenously injected horseradish peroxidase. J Cell Biol 20:497–507
Van Regenmortel MHV (1986) Which structural features determine protein antigenicity? TIBS 11:36–39
Yntema CL (1950) An analysis of induction of the ear from foreign ectoderm in the salamander embryo. J Exp Zool 113:211–244
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Mark, M.P., Prince, C.W., Gay, S. et al. 44-kDal bone phosphoprotein (osteopontin) antigenicity at ectopic sites in newborn rats: kidney and nervous tissues. Cell Tissue Res. 251, 23–30 (1988). https://doi.org/10.1007/BF00215443
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DOI: https://doi.org/10.1007/BF00215443