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Abundance and polymorphism of simple repetitive DNA sequences in Bmssica napus L.

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Summary

The Brassica napus genome has been investigated by DNA fingerprinting with six synthetic oligonucleotide probes complementary to simple repetitive sequences, namely (GATA)4, (GACA)4, (GGAT)4, (CA)8, (CT)8 and (GTG)5. While all sequence motifs were found to be present in the B. napus genome, their organization and abundance varied considerably. Among the investigated probes, (GATA)4 revealed the highest level of intraspecific polymorphism and distinguishes not only between cultivars but even between different individuals belonging to the same cultivar. In contrast, (GTG)5, (GACA)4 and (GGAT)4 produced relatively homogeneous fingerprint patterns throughout different cultivars, while hybridization to (CT)8 and (CA)8 resulted in only a few weak bands superimposed on a smear. The isoschizomeric pair Hpa II and Msp I revealed the presence of methylated cytosines in the vicinity of (GATA)m repeats. The applicability of simple repetitive sequence polymorphisms as molecular markers for Brassica species is discussed.

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References

  • Ali S, Müller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotide probes specific for simple repeats. Hum Genet 74:239–243

    Google Scholar 

  • Arus P, Shields CR, Orton TJ (1985) Application of isozyme electrophoresis for purity testing and cultivar identification of F1 hybrids of Brassica oleracea. Euphytica 34:651–657

    Google Scholar 

  • Belanger FC, Hepburn AG (1990) The evolution of CpNpG methylation in plants. J Mol Evol 30:26–35

    Google Scholar 

  • Beridze T (1975) DNA nuclear satellites of the genus Brassica: variation between species. Biochem Biophys Acta 395:274–279

    Google Scholar 

  • Berneman ZN, Raeymaekers P, Vanhoof G, Van Bockstaele D, Ramael M, Peetermans ME, Vanderberghe A, Van Broeckhoven C (1989) DNA fingerprints revealing common and divergent human DNA methylation patterns. FEBS Lett 255:226–230

    Google Scholar 

  • Beyermann B, Nürnberg P, Weihe A, Meixner M, Epplen JT, Börner T (1992) Fingerprinting plant genomes with oligonucleotide probes specific for simple repetitive sequences. Theor Appl Genet 83:691–694

    Google Scholar 

  • Bianchi MW, Viotti A (1988) DNA methylation and tissue-specific transcription of the storage protein genes of maize. Plant Mol Biol 11:203–214

    Google Scholar 

  • Epplen JT, Ammer H, Epplen C, Kammerbauer C, Mitreiter R, Roewer L, Schwaiger W, Steimle V, Zischler H, Albert E, Andreas A, Beyermann B, Meyer W, Buitkamp J, Nanda I, Schmid M, Nürnberg P, Pena SDJ, Pöche H, Sprecher W, Schartl M, Weising K, Yassouridis A (1991) Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA fingerprinting: approaches and applications. Birkhäuser, Basel, pp 50–69

    Google Scholar 

  • Evans DA (1989) Somaclonal variation — genetic basis and breeding applications. Trends Genet 5:46–50

    Google Scholar 

  • Grellet F, Delcasso D, Panabicrres F, Delseny M (1986) Organization and evolution of a higher plant alphoid-like satellite DNA sequence. J Mol Biol 187:495–507

    Google Scholar 

  • Gupta SK, Röbbelen G (1986) Identification of rapeseed (Brassica napus) cultivars by electrophoresis. Z Pflanzenzüchtg 96:363–370

    Google Scholar 

  • Gupta V, Jagannathan V, Lakshmikumaran MS (1990) A novel AT-rich tandem repeat of Brassica nigra. Plant Sci 68:223–229

    Google Scholar 

  • Halldén C, Bryngelsson T, Säll T, Gustafsson M (1987) Distribution and evolution of a tandemly repeated DNA sequence in the family Brassicaceae. J Mol Evol 25:318–323

    Google Scholar 

  • Harbinder S, Lakshmikumaran M (1990) A repetitive sequence from Diplotaxis erucoides is highly homologous to that of Brassica campestris and B. oleracea. Plant Mol Biol 15:155–156

    Google Scholar 

  • Hillel J, Schaap T, Haberfeld A, Jeffreys AJ, Plotzky Y, Cahaner A, Lavi U (1990) DNA fingerprints applied to gene introgression in breeding programs. Genetics 124:783–789

    Google Scholar 

  • Hofmann D, Weising K (1990) Extraction of ethidium bromide from CsCl-purified DNA. Focus 12:29

    Google Scholar 

  • Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511

    Google Scholar 

  • Iwabuchi M, Itoh K, Shimamoto K (1991) Molecular and cytological characterization of repetitive DNA sequences in Brassica. Theor Appl Genet 81:349–355

    Google Scholar 

  • Kessler C, Höltke HJ (1986) Specificity of restriction endonucleases and methylases — a review (edition 2). Gene 47:1–153

    Google Scholar 

  • Lakshmikumaran M, Ranade SA (1990) Isolation and characterization of a highly repetitive DNA of Brassica campestris. Plant Mol Biol 14:447–448

    Google Scholar 

  • Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34:543–552

    Google Scholar 

  • Levinson G, Gutman GA (1987) Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4:203–221

    Google Scholar 

  • Mündges H, Köhler W, Friedt W (1990) Identification of rape seed cultivars (Brassica napus) by starch-gel electrophoresis of enzymes. Euphytica 43:179–187

    Google Scholar 

  • Nybom H (1991) Applications of DNA fingerprinting in plant breeding. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA fingerprinting: approaches and applications. Birkhäuser, Basel, pp 294–311

    Google Scholar 

  • Poulsen GB, Nielsen SVS (1989) Regeneration of plants from hypocotyl protoplasts of rapeseed (Brassica napus L. var. oleifera) cultivars. Plant Cell Tissue Org Cult 17:153–158

    Google Scholar 

  • Prakash S, Hinata K (1980) Taxonomy, cytogenetics, and origin of crop Brassica, a review. Opera Bot 55:1–57

    Google Scholar 

  • Quiros CF, Ochoa O, Douches DS (1988) Exploring the role of x = 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Hered 79:351–358

    Google Scholar 

  • Quiros CF, Hu J, This P, Chevre AM, Delseny M (1991) Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica. Theor Appl Genet 82:627–632

    Google Scholar 

  • Reddy AS, Srivastava V, Guha-Mukherjee (1989) A tandemly repeated DNA sequence from Brassica juncea. Nucleic Acids Res 17:5849

    Google Scholar 

  • Riggs CD, Chrispeels MJ (1990) The expression of phytohemagglutinin genes in Phaseolus vulgaris is associated with organ-specific DNA methylation patterns. Plant Mol Biol 14:629–632

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Google Scholar 

  • Schäfer R, Zischler H, Birsner U, Becker A, Epplen JT (1988) Optimized oligonucleotide probes for DNA fingerprinting. Electrophoresis 9:369–374

    Google Scholar 

  • Schlötterer C, Tautz D (1992) Slippage synthesis of simple sequence DNA. Nucleic Acids Res 20:211–215

    Google Scholar 

  • Schmidt T, Junghans H, Metzlaff M (1990) Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines. Theor Appl Genet 79:177–181

    Google Scholar 

  • Schmidt T, Boblenz K, Metzlaff M, Kaemmer D, Weising K, Kahl G (1993) DNA fingerprinting in sugar beet (Beta vulgaris): identification of double-haploid breeding lines. Theor Appl Genet (in press)

  • Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor Appl Genet 75:679–684

    Google Scholar 

  • Selker EU (1990) DNA methylation and chromatin structure: a view from below. Trends Biochem Sci 15:103–107

    Google Scholar 

  • Sibson DR, Hughes SG, Bryant JA, Fitchett PN (1991) Sequence organization of simple, highly repetitive DNA elements in Brassica species. J Exp Bot 42:243–249

    Google Scholar 

  • Song K, Osborn TC, Williams PH (1990) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 3. Genome relationships in Brassica and related genera and the origin of B. Oleracea and B. Rapa (syn. campestris). Theor Appl Genet 79:497–506

    Google Scholar 

  • Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa syn. campestris based on restriction fragment length polymorphism loci. Theor Appl Genet 82:296–304

    Google Scholar 

  • Tautz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 12:4127–4136

    Google Scholar 

  • Thein SL, Wallace RB (1986) The use of synthetic oligo-nucleotides as specific hybridization probes in the diagnosis of genetic disorders. In: Davies KE (ed) Human genetic diseases — a practical approach. IRL Press, Oxford, pp 33–50

    Google Scholar 

  • Tzuri G, Hillel J, Lavi U, Haberfeld A, Vainstein A. (1991) DNA fingerprint analysis of ornamental plants. Plant Sci 76:91–97

    Google Scholar 

  • Van Heusden AW, van der Voort JR, Bachmann K (1991) Oligo-(GATA) fingerprints identify clones in asexual dan-delions Taraxacum (Asteraceae). Fingerprint News 3:13–15

    Google Scholar 

  • Van Houten WHJ, Van Heusden AW, Van der Voort JR, Raijmann L, Bachmann K (1991) Hypervariable DNA fingerprint loci in Microseris pygmaea (Asteraceae, Lactucaceae). Bot Acta 104:252–256

    Google Scholar 

  • Weising K, Beyermann B, Ramser J, Kahl G (1991a) Plant DNA fingerprinting with radioactive and digoxigenated oligo-nucleotide probes complementary to simple repetitive sequences. Electrophoresis 12:159–169

    Google Scholar 

  • Weising K, Ramser J, Kaemmer D, Kahl G, Epplen JT (1991b) Oligonucleotide fingerprinting in plants and fungi. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA fingerprinting: approaches and applications. Birkhäuser, Basel, pp 312–329

    Google Scholar 

  • Weising K, Kaemmer D, Weigand F, Epplen JT, Kahl G (1992) Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum). Genome 35:436–442

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Group, Danish Research Service for Plant and Soil Science, Lottenborgvej 2, DK-2800, Lyngby, Denmark

    G. B. Poulsen

  2. Plant Molecular Biology Group, University of Frankfurt, Siesmayerstrasse 70, D-W-6000, Frankfurt am Main, FRG

    G. Kahl & K. Weising

Authors
  1. G. B. Poulsen
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  2. G. Kahl
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  3. K. Weising
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Communicated by G. Wenzel

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Poulsen, G.B., Kahl, G. & Weising, K. Abundance and polymorphism of simple repetitive DNA sequences in Bmssica napus L.. Theoret. Appl. Genetics 85, 994–1000 (1993). https://doi.org/10.1007/BF00215039

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  • DOI: https://doi.org/10.1007/BF00215039

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