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The use of gelatin as a vehicle for drug and peptide delivery

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Abstract

Gelatin, a naturally occurring polymer, has been investigated as a vehicle for drug delivery in two different delivery systems: microspheres and as a coating on titanium implants. The gelatin was loaded with recombinant human growth hormone (hGH) which was dispersed within the polymer matrix prior to crosslinking; it was then made into microspheres or coated onto the implants. The release of hGH was monitored in vitro using an “in-house” ELISA system. The effects of pH on the swelling kinetics and the physical properties of the loaded gelatin in the microsphere system were studied. In addition, the effect of ultrasound on the microspheres was investigated as a possible method for controlling the rate of release of hGH, it was demonstrated that exposure to ultrasound significantly increased hGH release. Biocompatibility of the gelatin was determined using both primary human (HOB) and rabbit (ROB) osteoblast-like cells in culture.

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References

  1. S.MOHAN and D. J.BAYLINK, Clin. Orthop. Rel. Res. 263 (1991) 30.

    Google Scholar 

  2. E.CANALIS, T.MCCARTHY and M.CENTRELLA, J. Clin. Invest. 81 (1988) 277.

    Google Scholar 

  3. B. A. A.SCHEVEN, N. J.HAMILTON, T. M. V.FAKKELDIJ and S. A.DUURSMA, Growth Regulation 1 (1991) 160

    Google Scholar 

  4. G.MOREL, B.CHAVASSIEUX, B.BARENTON, P. M.DUBOIS, P. J.MEUNIER and G.BOIVIN, Cell Tiss. Res. 273 (1993) 279.

    Google Scholar 

  5. M.KASSEM, W.BLUM, J.RISTELLI, L.MOSEKILDE and E. F.ERIKSEN, Calcif. Tiss. Int. 52 (1993) 222.

    Google Scholar 

  6. S.DOWNES, D.WOOD, A. J.MALCOLM and S. Y.ALI, Clin. Orth. Rel. Res. 252 (1990) 294.

    Google Scholar 

  7. S.DOWNES, M. V.KAYSER, G.BLUNN and S. Y.ALI, Cells Mater. 1 (1991) 171.

    Google Scholar 

  8. S.DOWNES, L.DISILVIO, C. A. P. T.KLIEN and M. V.KAYSER, J. Mater. Sci. Mater. Med. 2 (1991) 176.

    Google Scholar 

  9. S.DOWNES, L.DISILVIO, R. S.ARCHER and M. V.KAYSER, in “Bone-bonding”, edited by P.DUCHEYENE, T.KOKUBO and C. A.VANBLITTERSWIJK (Reed Healthcare Communications, Leiden, The Netherlands, 1992) p. 101.

    Google Scholar 

  10. S. DOWNES, C. J. CLIFFORD, K. DAVY and M. BRADEN, in Proceedings of International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society Inc. 19 (1992) 204.

  11. H. B. HOFFENBERG, “Controlled release polymeric formulations”, ACS Symposium Series, 33 (1976) p. 26.

  12. C. J. KIM and P. I. LEE, in Proceed. Intern. Symp. Control. Rel. Bioact. Mater. Controlled Release Society, Inc. 19 (1992) 208

  13. R.GALE, S. K.CHANDRASEKARAN, D.SWANSON and J.WRIGHT, J. Membrane Sci. 7 (1980) 319.

    Google Scholar 

  14. T. J. ROSEMAN and S. H. YALKOWSKY, “Controlled release polymeric formulations”, ACS Symposium Series, 33 (1976) p. 33.

  15. N. A.PEPPAS and R.LANGER, Science 263, (1994) 1715.

    Google Scholar 

  16. I. L.PING and N. A.PEPPAS, J. Controlled Release 6 (1987) 207.

    Google Scholar 

  17. R.GURNY, E.DOELKER and N. A.PEPPAS, Biomaterials 3, (1982) 27.

    Google Scholar 

  18. R. LANGER, S. T. HSIEH, S. T. DEAN, A. PEIL, R. BAWA and W. RHINE, “Controlled release system”, AIChE Symposium Series, 77 (206) (1981) p. 10.

  19. J.HELLER, D. W. H.PENHALE, R. F.HELWING, B. K.FRITZINGER and R. W.BAKER, “Controlled release systems”, AIChE Symposium Series, 77 (206) (1981) 28.

    Google Scholar 

  20. R.NARAYANI and K. P.RAO, Microencapsulation 11 (1994) 69.

    Google Scholar 

  21. F.FORNI, M. G.VANDELLI and R. J.CAMERON, Microencapsulation 9 (1992) 29.

    Google Scholar 

  22. G.GREGORIADIS and A. T.FLORENCE, Drugs (Review Article) 45 (1993) 15.

    Google Scholar 

  23. J. A.BAKAN, in “Polymers in medicine and surgery-microcapsule drug delivery system”, Poly. Science Tecnol. 8, edited by R. L.KRONENTHAL, Z.OSER and E.MARTIN (Plenum, London, 1975) p. 213.

    Google Scholar 

  24. J. K.RAO, D. V.RAMESH and K. P.RAO, Biomaterials 15 (1994) 383.

    Google Scholar 

  25. L. M.SANDERS, G. I.MCRAE, K. M.VITALE and B. A.KELL, J. Controlled Release 2 (1985) 187.

    Google Scholar 

  26. T. R. TICE, D. W. MASON and R. M. GILLEY, in “Novel drug delivery and its therapeutic application”, edited by L. F. Prescott and W. S. Nimmo, (Wiley, 1989) p. 223.

  27. P. J.PRINGLE, L.DISILVIO, P. C.HINDMARSH, R.MATTHEWS, A. B.KURTZ and C. G. D.BROOK, Clin. Endocrinol. 37 (1992) 169.

    Google Scholar 

  28. P. A.EALEY, M. E.YATEMAN, S. J.HOLT and N. J.MARSHALL, J. Molecular Endo. 1 (1988) R1-R4.

    Google Scholar 

  29. M. J.PIKAL, K.DELLERMAN and M. L.ROY, Dev. Biol. Stand. 74 (1922) 21.

    Google Scholar 

  30. M. J.PIKAL, K.DELLERMAN and EliLILLY and CO. Pharm. Res. 8 (1991) 427.

    Google Scholar 

  31. D. N.BREMS, P. L.BROWN and G. W.BECKER, J. Biol. Chem. 265 (1990) 5504.

    Google Scholar 

  32. A. W.PURCELL, M. I.AGUILAR and M. T.HEARN, J. Chromatog. 476 (1989) 125.

    Google Scholar 

  33. R. C.CLARK and A.COURTS, in “The science and technology of gelatin” Food and Tecnology Series, edited by A. G.WARD and A.COURTS (Academic, London, 1977) p. 209.

    Google Scholar 

  34. L.DISILVIO, N.GURAV, M. V.KAYSER, M.BRADEN and S.DOWNES, Biomaterials 15 (1994) 931.

    CAS  PubMed  Google Scholar 

  35. J.KOST, K.LEONG and R.LANGER, Proc. Natl Acad. Sci. USA. 86 (1989) 7663.

    Google Scholar 

  36. J.KOST, Clin. Mater. 13 (1993) 155.

    Google Scholar 

  37. P. C.HINDMARSH, D. R.MATTHEWS, C. E.BRAIN, P. J.PRINGLE, L.DISILVIO, A. B.KURTZ and C. G. D.BROOK, Clin. Endocrinol. 30 (1989) 443.

    Google Scholar 

  38. C. P. A. T.KLIEN, P.PATKA, J. G. C.WOLKE, J. M. A.DEBLIECK-HOGERVORST and K.DEGROOT, Biomaterials 15 (1994) 146.

    Google Scholar 

  39. F. W.COOKE, Clin. Orthop. Rel. Res. 276 (1992) 135.

    Google Scholar 

  40. J. E.LEMONS, Clin. Orthop. Rel. Res. 235 (1988) 220.

    Google Scholar 

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Authors and Affiliations

  1. IRC in Biomedical Materials, Institute of Orthopaedics (UCL), Royal National Orthopaedic Hospital Trust, Brockley Hill, HA7 4LP, Stanmore, Middlesex, UK

    L. Di Silvio, R. G. Courteney-Harris & S. Downes

Authors
  1. L. Di Silvio
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  2. R. G. Courteney-Harris
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  3. S. Downes
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Di Silvio, L., Courteney-Harris, R.G. & Downes, S. The use of gelatin as a vehicle for drug and peptide delivery. J Mater Sci: Mater Med 5, 819–823 (1994). https://doi.org/10.1007/BF00213141

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