Abstract
A non-green catabolite of chlorophyll (Chl) the fluorescent compound FC 2, is produced when intact senescent chloroplasts of barley (Hordeum vulgare L.) are incubated in the presence of ATP. The origin of FC 2 has now been demonstrated by employing senescent chloroplasts containing Chl 14C-labelled in the pyrrole-rings: upon incubation in the presence of ATP, 14C-labelled FC 2 is generated. The production of FC 2 requires the hydrolysis of ATP as demonstrated by the failure of the β, γ-imido analogue to support the reaction. Adenosine triphosphate can partially be replaced by UTP but GTP and CTP, as well as ADP and AMP, are ineffective. The system responsible for FC 2 production can also be fueled with glucose-6-phosphate, fructose-6-phosphate and glucose-1-phosphate; other sugar-phosphates including glyceraldehyde-3-phosphate have no effect. Adenosine triphosphate is also required for the release of FC 2 from chloroplasts. When chloroplasts are incubated in the presence of UTP or hexose-monophosphates which support the generation of FC 2 within the organelles, the catabolite is not released. It is concluded that the envelope of senescent chloroplasts is equipped with translocators for the cytosolic compounds which provide the metabolic energy and cofactors required for the action of the catalyst(s) responsible for the oxidative cleavage of Chl-porphyrin and possibly also for the dismantling of Chl-protein complexes. Moreover, a translocator may be involved in the release of the primary catabolites of Chl from chloroplasts.
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Abbreviations
- Chl:
-
chlorophyll
- Glc6P:
-
glucose-6-phosphate
- FC:
-
fluorescent compound
- HPLC:
-
high-performance liquid chromatography
References
Abeles, F.B., Dunn, L.J. (1989) Role of peroxidase during ethylene-induced chlorophyll breakdown in Cucumis sativus cotyledons. J. Plant Growth Regul. 8, 319–326
Bortlik, K., Peisker, C., Matile, Ph. (1990) A novel type of chlorophyll catabolite in senescent barley leaves. J. Plant Physiol. 136, 161–165
Düggelin, Th., Bortlik, K. Gut, H., Matile, Ph., Thomas, H. (1988a) Leaf senescence in Festuca pratensis: Accumulation of lipofuscin-like compounds. Physiol. Plant. 74, 131–136
Düggelin, Th., Schellenberg, M., Bortlik, K., Matile Ph. (1988b) Vacuolar location of lipofuscin- and proline-like compounds in senescent barley leaves. J. Plant Physiol. 133, 492–497
Hendry, G.A.F., Houghton, J.D., Brown, S.B. (1987) The degradation of chlorophyll — a biological enigma. New Phytol. 107, 255–302
Holden, M. (1965) Chlorophyll bleaching by legume seeds (1965) J. Sci. Food Agric. 16, 312–325
Huff, A. (1982) Peroxidase-catalysed oxidation of chlorophyll by hydrogen peroxide. Phytochemistry, 21, 261–265
Kato, M., Shimizu, S. (1985) Chlorophyll metabolism in higher plants — 6.Involvement of peroxidase in chlorophyll degradation. Plant Cell Physiol. 26, 1291–1301
Kräutler, B., Jaun, B., Bortlik, K., Schellenberg, M., Matile, Ph. (1991) Zum Rätsel des Chlorophyllabbaues. — Die Konstitution eines secoporphinoiden Kataboliten. Angew. Chem. 103, 1354–1357
Lichtenthaler, H.K., Wellburn, A.R. (1983) Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11, 591–592
Lüthy, B., Thomas, H., Matile, Ph. (1986) Linolenic acid-dependent “chlorophyll oxidase”-activity: a property of photosystems I and II. J. Plant Physiol. 123, 203–209
Makovetski, S., Goldschmidt, E.E. (1976) A requirement for cytoplasmic protein synthesis during chloroplast senescence in the aquatic plant Anacharis canadensis. Plant Cell Physiol. 17, 859–862
Martinoia, E., Dalling, M.J., Matile, Ph. (1982) Catabolism of chlorophyll: demonstration of chloroplast-localized peroxidative and oxidative activities. Z. Pflanzenphysiol. 107, 269–279
Matile, Ph. (1980) Catabolism of chlorophyll: Involvement of peroxidase? Z. Pflanzenphysiol. 99, 475–478
Matile, Ph., Düggelin, T., Schellenberg, M., Rentsch, D., Bortlik, K., Peisker, C., Thomas, H. (1989) How and why is chlorophyll broken down in senescent leaves? Plant Physiol. Biochem. 27, 595–604
Peisker, C., Thomas, H., Keller, F., Matile, Ph. (1990) Radiolabelling of chlorophyll for studies of catabolism. J. Plant Physiol. 136, 544–549
Sakai-Imamura, M. (1975) Fatty acid oxidation and chlorophyll bleaching. Nat. Sci. Rep. Ochanomizu Univ. 26, 109–125
Schellenberg, M., Matile, Ph., Thomas, H. (1990) Breakdown of chlorophyll in chloroplasts of senescent barley leaves depends on ATP. J. Plant Physiol. 136, 564–568
Schoch, S., Brown, J. (1987) The action of chlorophyllase on chlorophyll-protein complexes. J. Plant Physiol. 126, 483–494
Sitte, P., Falk, H., Liedvogel, B. (1980) Chromoplasts. In: Pigments in plants, pp. 117–148, Czygan, F.-C., ed. Gustav Fischer, Stuttgart New York
Thomas, H. (1976) Delayed Senescence in leaves treated with protein synthesis inhibitor MDMP. Plant Sci. Lett. 6, 369–378
Thomas, H., Bortlik, K., Rentsch, D., Schellenberg, M., Matile, Ph. (1989) Catabolism of chlorophyll in vivo: significance of polar chlorophyll catabolites in a non-yellowing senescence mutant of Festuca pratensis Huds. New Phytol. 111, 3–8
Wagner, W., Keller, F., Wiemken, A. (1983) Fructan metabolism in cereals: induction in leaves and compartmentation in protoplasts and vacuoles. Z. Pflanzenphysiol. 112, 359–372
Yamauchi, N., Minamide, T. (1985) Chlorophyll degradation by peroxidase in parsley leaves. J. Jpn. Soc. Hort. Sci. 54, 265–271
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We are indebted to the Swiss National Science Foundation as well as to Jubiläumsstiftung der Universität Zürich for financial support, and to H. Müller for help with the manuscript.
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Matile, P., Schellenberg, M. & Peisker, C. Production and release of a chlorophyll catabolite in isolated senescent chloroplasts. Planta 187, 230–235 (1992). https://doi.org/10.1007/BF00201944
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DOI: https://doi.org/10.1007/BF00201944