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Glycosyl-phosphatidylinositol-anchored proteins exist in the plasma membrane of Chlorella saccharophila (Krüger) Nadson: Plasma-membrane-bound nitrate reductase as an example

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Abstract

Experiments with plasma-membrane vesicles were performed in order to identify the attachment of hydrophobic nitrate reductase at the plasma membrane of Chlorella saccharophila. The enzyme was successfully removed from the plasma membrane with phosphoinositol-specific phospholipase C, and showed cross-reactivity with a monoclonal antibody (clone aGPI-3) raised against the glycosyl-phosphatidylinositol (GPI) anchor of Trypanosoma variant surface protein. The enzyme was labelled in vivo by feeding [3H]ethanolamine to the cells and underwent an hydrophobicity shift after treatment with phosphoinositol-specific phospholipase C. The attachment of this form of nitrate reductase to the plasma membrane via a GPI anchor was demonstrated.

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Abbreviations

GPI:

glycosyl-phosphatidylinositol

NR:

nitratereductase

PI-PLC:

phosphoinositol-specific phospholipase C

PMNR:

Plasma-membrane-bound nitrate reductase

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Authors and Affiliations

  1. Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany

    Christine Stöhr & Rudolf Tischner

  2. Physiologisch-Chemisches Institut, Universität Bonn, Nussallee 11, D-53115, Bonn, Germany

    Frank Schuler

Authors
  1. Christine Stöhr
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  2. Frank Schuler
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  3. Rudolf Tischner
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Additional information

The research was supported by a grant from Deutsche Forschungsgemeinschaft to R.T.

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Stöhr, C., Schuler, F. & Tischner, R. Glycosyl-phosphatidylinositol-anchored proteins exist in the plasma membrane of Chlorella saccharophila (Krüger) Nadson: Plasma-membrane-bound nitrate reductase as an example. Planta 196, 284–287 (1995). https://doi.org/10.1007/BF00201386

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  • DOI: https://doi.org/10.1007/BF00201386

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