Abstract
Stimulation of G-protein activity by the amphipathic tetradecapeptide mastoparan is well documented in animal systems and occurs via mimickry of the third cytoplasmic domain of the cognate seven-transmembrane-span (7TMS) receptor. Binding of guanosine 5′-O-thiotriphosphate to microsomal and plasma-membrane fractions from Pisum sativum L. and Zea mays L. was stimulated by the tetradecapeptide mastoparan analogue mas 7 over a narrow concentration range, with maximal effect exerted at 10 μM peptide, while the nonamphipathic analogue, mas CP, which differed from mas 7 by only one amino acid, was ineffective at promoting binding. This stimulation could be completely abolished by inclusion of low concentrations of the non-ionic detergents nonanoyl-N-methylglucamide and Lubrol PX. Taken together, these data provide good evidence for the existence of G-protein-linked 7TMS receptors in higher plant systems.
Similar content being viewed by others
Abbreviations
- GTPγS:
-
guanosine 5′-O-thiotriphosphate
- MEGA-9:
-
nonanoyl-N-methylglucamide
- 7TMS:
-
7 trans-membrane span
References
Atherton, E., Sheppard, R.C. (1989) Fluorenylmethoxycarbonyl-polyamide solid phase peptide synthesis-general principles and development. In: Solid phase peptide synthesis, a practical approach, pp. 25–37, Atherton, E., Sheppard, R.C., eds. IRL Press, Oxford
Birnbaumer, L., Abramowitz, J., Brown, A.M. (1990) Receptor-effector coupling by G-proteins. Biochim. Biophys. Acta 1031, 163–224
Birnbaumer, L. (1992) Receptor-to-effector signalling through G-proteins: Roles for βγ dimers as well as α subunit. Cell 71, 1069–1072
Dillenschneider, M., Hetherington, A., Graziana, A., Alibert, G., Haiech, J. Ranjeva, R. (1986) The formation of inositol phosphate derivatives by isolated membranes from Acer pseudoplatanus is stimulated by guanine nucleotides. FEBS Lett. 208, 413–417
Fairley-Grenot, K., Assman, S.M. (1991) Evidence for G-protein regulation of inward K+ channel current in guard cells of fava bean. Plant Cell, 3, 1037–1044
Hamm, H.E., Deretic, D., Arendt, A., Hargrave, P.A., Koenig, B., Hoffman, K.P. (1988) Site of G-protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit. Science 241, 832–835
Hepler, J.R., Gilman, A.G. (1992) G-proteins. Trends. Biochem. Sci. 17, 383–387
Higashijima, T., Uzu, S., Nakajima, T., Ross, E.M. (1988) Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins. J. Biol. Chem. 263, 6491–6494
Higashijima, T., Burnier, J., Ross, E.M. (1990) Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity. J. Biol. Chem. 265, 14176–14186
Hirai, Y., Yasuhara, T., Yoshisda, H., Nakajima, T., Fujino, M., Kitada, C. (1979) A new mast cell degranulating peptide “mastoparan” in the venom of Vespula lewisii. Chem. Pharm. Bull. 27, 1942–1944
Kikkawa, S., Takahashi, K., Takahashi, K., Shimada, N., Ui, M., Kimura, N., Katada, T. (1992) Activation of nucleoside diphosphate kinase by mastoparan, a peptide isolated from wasp venom. FEBS Lett. 305, 237–240
Kjellbom, P., Larsson, C. (1984) Preparation and polypeptide composition of chlorophyll-free plasma membranes from leaves of light grown spinach and barley. Physiol. Plant. 62, 501–509
Larsson, C., Widdell, S., Kjellbom, P. (1987) Preparation of high purity plasma membranes. Methods Enzymol. 148, 558–568
Li, W., Assmann, S.M. (1993) Characterization of a G-proteinregulated outward K+ current in mesophyll cells of Vicia faba L. Proc. Natl. Acad. Sci. USA. 90, 262–266
Ma, H., Yanofsky, M.F., Meyerowitz, E.M. (1990) Molecular cloning and characterisation of GPA1, a G protein α subunit. Proc. Natl. Acad. Sci. USA. 87, 3821–3825
Ma, H., Yanofsky, M.F., Huang, H. (1991) Isolation and sequence analysis of TGA1 cDNAs encoding a tomato G protein α subunit. Gene 107, 189–195
Northup, J.K., Smigel, M.D., Gilman, A.G. (1982) The guanine nucleotide activating site of the regulatory component of adenylate cyclase. J. Biol. Chem. 257, 11416–11423
Oppi, C., Wagner, T., Crisari, A., Camerini, B., Tocchini Valentini, G-P. (1992) Attenuation of GTPase activity of recombinant Goα by peptides representing sequence permutations of mastoparan. Proc. Natl. Acad. Sci. USA. 89, 8268–8272
Palm, D., Munch, G., Dees, C., Hekman, M. (1989) Mapping of β-adrenoreceptor coupling domains to Gs-protein by site-specific synthetic peptides. FEBS Lett. 254, 89–93
Palm, D., Munch, G., Malek, D., Dees, C., Hekman, M. (1990) Identification of a Gs protein coupling domain to the betaadrenoreceptor using site specific synthetic peptides. FEBS Lett. 261, 294–298
Romero, L.C., Sommer, D., Gotor, C., Song, P.-S. (1991) G-proteins in etiolated Avena seedlings. Possible phytochrome regulation. FEBS Lett. 282, 341–346
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85
Sullivan, K.A., Miller, R.T., Masters, S.B., Beiderman, B., Heiderman, W., Bourne, H.R. (1987) Identification of receptor contact site involved in receptor — G-protein coupling. Nature 330, 758–760
Ui, M. (1984) Islet activating protein, pertussis toxin: A probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. Trends. Pharmacol. Sci. 5, 277–279
Warpeha, K.M.F., Hamm, H.E., Rasenik, M.M., Kaufman, L.S. (1991) A blue-light-activated GTP-binding protein in the plasma membranes of etiolated peas. Proc. Natl. Acad. Sci. USA. 88, 8925–8929
Warpeha, K.M.F., Kaufman, L.S., Briggs, W.R. (1992) A flavoprotein may mediate the blue light-activated binding of guanosine 5′ triphosphate to isolated plasma membranes of Pisum sativum L. Photochem. Photobiol. 55, 595–603
Weingarten, R., Ransnäs, L., Mueller, H., Sklar, L.A., Bokoch, G.M. (1990) Mastoparan interacts with the carboxyl terminus of the α subunit of Gi. J. Biol. Chem. 265, 11044–11049
West, R.E., Moss, J., Vaughan, M., Liu, T., Liu, T-Y. (1985) Pertussis toxin catalysed ADP-ribosylation of transducin. J. Biol. Chem. 260, 14428–14430
White, I.R., Wise, A., Finan, P.M., Clarkson, J., Millner, P.A. (1992) GTP-binding proteins in higher plant cells. In: Transport and receptor proteins of plant membranes, pp. 185–192, Cooke, D.T., Clarkson, D.T., eds. Plenum Press, New York
White, I.R., Finan, P.M., Millner, P.A. (1993) Nucleoside diphosphate kinase associated with Pisum sativum microsomal membranes: Apparent binding of GTPγS at nM concentrations. J. Plant Physiol., in press
Zaina, S., Reggiani, R., Alcide, B. (1990) Preliminary evidence for the involvement of GTP-binding proteins in rice (Oryzasativa L.) coleoptile. J. Plant Physiol. 136, 653–658
Zbell, B., Walter-Back, C. (1988) Signal transduction of auxin on isolated plant cell membranes — indications for a rapid polyphosphoinositide response stimulated by indole acetic acid. J. Plant. Physiol. 133, 353–360
Author information
Authors and Affiliations
Additional information
The authors wish to acknowledge the financial support of the European Community (I.R.W.), ICI and the Science and Engineering Research Council (A.W.) and the Agricultural and Food Research Council (P.A.M.).
Rights and permissions
About this article
Cite this article
White, I.R., Wise, A. & Millner, P.A. Evidence for G-protein-linked receptors in higher plants: stimulation of GTP-gamma-S binding to membrane fractions by the mastoparan analogue mas 7. Planta 191, 285–288 (1993). https://doi.org/10.1007/BF00199762
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00199762