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Polyphosphoinositol lipids in Chlamydomonas eugametos gametes

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Abstract

In Chlamydomonas eugametos gametes, phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol 4,5-bisphosphate (PtdInsP2) comprised 0.4 and 0.3% of the whole-cell phospholipids. They were concentrated in the plasma membrane around the cell body and were present in low concentrations in the flagellar membrane. When gametes were fed 32PO -4 , the label was rapidly incorporated into PtdInsP and PtdInsP2 and only slowly incorporated into structural lipids such as phosphatidylethanolamine and phosphatidylglycerol. Similarly, when a pulse of 32PO -4 was chased with PO -4 , the label was rapidly lost from the polyphosphoinositol lipids but not from the structural lipids. The major fatty acids in the polyphosphoinositides were C-22 carbon polyenoic acids (70%). The significance of these results in relationship to intracellular signalling via inositol phosphates and Ca2+ is discussed.

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Abbreviations

InsP3 :

inositol 1,4,5-trisphosphate

mt−/mt+ :

mating-type plus or minus

PtdA:

phosphatidic acid

PtdEtn:

phosphatidylethanolamine

PtdGro:

phosphatidylglycerol

PtdIns:

phosphatidylinositol

PtdInsP:

phosphatidylinositol 4-phosphate;

PtdInsP2 :

phosphatidylinositol 4,5-bisphosphate

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098, SM Amsterdam, The Netherlands

    Jeanine Brederoo, Piet de Wildt, Alan Musgrave & Herman van den Ende

  2. Department of Endocrinology, Free University Hospital, NL-1098, Amsterdam, The Netherlands

    Corrie Popp-Snijders

  3. Department of Biochemistry, Institute of Animal Physiology and Genetics Research, CB2 4AT, Babraham, Cambridge, UK

    Robin F. Irvine

Authors
  1. Jeanine Brederoo
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  2. Piet de Wildt
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  3. Corrie Popp-Snijders
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  4. Robin F. Irvine
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  5. Alan Musgrave
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  6. Herman van den Ende
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Additional information

We thank Frank Schuring for Fig. 5A and Susan Kenter, Hans Kruisselbrink, Saskia Bijvank and Nelleke Corbett for their enthousiastic assistance.

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Brederoo, J., de Wildt, P., Popp-Snijders, C. et al. Polyphosphoinositol lipids in Chlamydomonas eugametos gametes. Planta 184, 175–181 (1991). https://doi.org/10.1007/BF00197945

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