Abstract
In Chlamydomonas eugametos gametes, phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol 4,5-bisphosphate (PtdInsP2) comprised 0.4 and 0.3% of the whole-cell phospholipids. They were concentrated in the plasma membrane around the cell body and were present in low concentrations in the flagellar membrane. When gametes were fed 32PO -4 , the label was rapidly incorporated into PtdInsP and PtdInsP2 and only slowly incorporated into structural lipids such as phosphatidylethanolamine and phosphatidylglycerol. Similarly, when a pulse of 32PO -4 was chased with PO -4 , the label was rapidly lost from the polyphosphoinositol lipids but not from the structural lipids. The major fatty acids in the polyphosphoinositides were C-22 carbon polyenoic acids (70%). The significance of these results in relationship to intracellular signalling via inositol phosphates and Ca2+ is discussed.
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Abbreviations
- InsP3 :
-
inositol 1,4,5-trisphosphate
- mt−/mt+ :
-
mating-type plus or minus
- PtdA:
-
phosphatidic acid
- PtdEtn:
-
phosphatidylethanolamine
- PtdGro:
-
phosphatidylglycerol
- PtdIns:
-
phosphatidylinositol
- PtdInsP:
-
phosphatidylinositol 4-phosphate;
- PtdInsP2 :
-
phosphatidylinositol 4,5-bisphosphate
- TCA:
-
trichloroacetic acid
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We thank Frank Schuring for Fig. 5A and Susan Kenter, Hans Kruisselbrink, Saskia Bijvank and Nelleke Corbett for their enthousiastic assistance.
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Brederoo, J., de Wildt, P., Popp-Snijders, C. et al. Polyphosphoinositol lipids in Chlamydomonas eugametos gametes. Planta 184, 175–181 (1991). https://doi.org/10.1007/BF00197945
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DOI: https://doi.org/10.1007/BF00197945