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Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues

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Abstract

An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.

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Abbreviations

AG:

1-aminoguanidine

AT:

3-amino-1,2,4-triazole

β-HEH:

β-hydroxyethylhydrazine

DAO(s):

diamine oxidase(s)

PAO(s):

polyamine oxidase(s)

Put:

putrescine

Spd:

spermidine

Spm:

spermine

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Authors and Affiliations

  1. Department of Biology, Williams College, 01267, Williamstown, MA, USA

    R. D. Slocum & M. J. Furey III

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  1. R. D. Slocum
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  2. M. J. Furey III
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Additional information

The authors wish to thank Nancy Piatczyc for the technical assistance with electron-microscopy studies. We are grateful to Dr. Stanley J. Roux, University of Texas at Austin, for providing us with samples of maize cell-wall exudates. This work was supported by grants to R.D.S from the National Aeronautics and Space Administration (NAGW-1049 and NAGW-1382).

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Slocum, R.D., Furey, M.J. Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues. Planta 183, 443–450 (1991). https://doi.org/10.1007/BF00197744

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  • DOI: https://doi.org/10.1007/BF00197744

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