Abstract
The relationship between growth and increase in cell-wall material (wall synthesis) was investigated in hypocotyls of sunflower seedlings (Helianthus annuus L.) that were either grown in the dark or irradiated with continuous white light (WL). The peripheral three to four cell layers comprised 30–50% of the entire wall material of the hypocotyl. The increase in wall material during growth in the dark and WL, respectively, was larger in the inner tissues than in the peripheral cell layers. The wall mass per length decreased continuously, indicating that wall thinning occurs during growth of the hypocotyl. When dark-grown seedlings were transfered to WL, a 70% inhibition of growth was observed, but the increase in wall mass was unaffected. Likewise, the composition of the cell walls (cellulose, hemicellulose, pectic substances) was not affected by WL irradiation. Upon transfer of dark-grown seedlings into WL a drastic increase in wall thickness and a concomitant decrease in cell-wall plasticity was measured. The results indicate that cell-wall synthesis and cell elongation are independent processes and that, as a result, WL irradiation of etiolated hypocotyls leads to a thickening and mechanical stiffening of the cell walls.
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Abbreviations
- IT:
-
inner tissues
- OT:
-
outer tissues
- WL:
-
white light
References
Brummell, D. A., Hall, J.L. (1987) Rapid cellular responses to auxin and the regulation of growth. Plant Cell Environ. 10, 523–543
Burström, H.G. (1979) In search of a plant growth paradigm. Am.J. Bot. 66, 98–104
Carpita, N.C. (1984) Cell wall development in maize coleoptiles. Plant Physiol. 76, 205–212
Carrington, C.M.S., Firn, R.D. (1985) Polysaccharide synthesis and turnover in the cell walls of growing and non-growing cells of gravistimulated sunflower hypocotyls. J. Plant Physiol. 118, 49–59
Cosgrove, D.J. (1988) Mechanism of rapid supression of cell expansion in cucumber hypocotyls after blue-light irradiation. Planta 176, 109–116
Edelmann, M., Bergfeld, R., Schopfer, P. (1989) Role of cell-wall biogenesis in the initiation of auxin-mediated growth in coleoptiles of Zea mays L. Planta 179, 486–494
Fry, S.C. (1988) The growing plant cell wall: chemical and metabolic analysis. Longman, London & Wiley, New York
Gessner, F. (1934) Wachstum und Wanddehnbarkeit im Helianthus-Hypocotyl. Jahrb. Wiss. Bot. 80, 143–168
Heyn, A.N.J. (1931) Der Mechanismus der Zellstreckung. Rec. Trav. Bot. Neerl. 28, 113–244
Heyn, A.N.J. (1940) The physiology of cell elongation. Bot. Rev. 6, 515–574
Kutschera, U. (1989) Tissue stresses in growing plant organs. Physiol. Plant. 77, 157–163
Kutschera, U., Briggs, W.R. (1987a) Rapid auxin-induced stimulation of cell wall synthesis in pea internodes. Proc. Natl. Acad.Sci. USA 84, 2747–2751
Kutschera, U., Briggs, W.R. (1987b) Differential effect of auxin on in vivo extensibility of cortical cylinder and epidermis in pea internodes. Plant Physiol. 84, 1361–1366
Kutschera, U., Kende, H. (1988) The biophysical basis of elongation growth in internodes of deepwater rice. Plant Physiol. 88, 361–366
Kutschera, U., Bergfeld, R., Schopfer, P. (1987) Cooperation of epidermis and inner tissues in auxin-mediated growth of maize coleoptiles. Planta 170, 168–180
Lockhart, J. (1960) Intracellular mechanism of growth inhibition by radiant energy. Plant Physiol. 35, 129–135
Lockhart, J. (1965) Cell extension. In: Plant biochemistry, pp. 826–849, Bonner, J., Varner, J.E., eds. Academic Press, New York
McNeil, M., Darvill, A.G., Fry, S.C., Albersheim, P. (1984) Structure and function of the primary cell walls of plants. Annu. Rev. Biochem. 53, 625–663
Meinert, M.C., Delmer, D.P. (1977) Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol. 59, 1088–1097
Mohr, H. (1972) Lectures on photomorphogenesis. Springer, Berlin Heidelberg New York Tokyo
Pfeffer, W. (1893) Druck-und Arbeitsleistung durch wachsende Pflanzen. Abh. Königl. Sächs. Ges. Wiss. 33, 235–474
Phillips, H.L., Kende, H. (1980) Structural changes of flowers of Ipomoea tricolor during flower opening and closing. Protoplasma 102, 199–215
Preston, R.D. (1974) The physical biology of plant cell walls. Chapman and Hall, London
Ray, P.M. (1962) Cell wall synthesis and cell elongation in oat coleoptile tissue. Am. J. Bot. 49, 928–939
Ray, P.M. (1967) Radioautographic study of cell wall deposition in growing plant cells. J. Cell Biol. 35, 659–674
Ray, P.M. (1987) Principles of plant cell growth. In: Physiology of cell expansion during plant growth, pp 1–17, Cosgrove, D.J., Knievel, D.P., eds. American Society of Plant Physiologists, Rockville Md. USA
Roland, J.-C., Reis, D., Mosiniak, M., Vian, B. (1982) Cell wall texture along the growth gradient of the mung bean hypocotyl: ordered assembly and dissipative processes. J. Cell Sci. 56, 303–318
Sargent, J.A., Atack, A.V., Osborne, D.J. (1974) Auxin and ethylene control of growth in epidermal cells of Pisum sativum: a biphasic response to auxin. Planta 115, 213–225
Schnepf, E., Deichgräber, G. (1979) Elongation growth of setae of Pellia (Bryophyta): fine structure analysis. Z. Pflanzenphysiol. 94, 283–297
Spurr, A.P. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43
Taiz, L. (1984) Plant cell expansion: regulation of cell wall mechanical properties. Annu. Rev. Plant Physiol. 35, 585–657
Updegraff, D.M. (1969) Semimicro determination of cellulose in biological materials. Anal. Biochem. 32, 420–424
Varner, J.E., Lin, L.-S. (1989) Plant cell wall architecture. Cell 56, 231–239
Wakabayashi, K., Sakurai, N., Kuraishi, S. (1989) Role of the outer tissue in abscisic acid-mediated growth suppression of etiolated squash hypocotyl segments. Physiol. Plant. 75, 151–156
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I wish to thank Professor A. Sievers for providing the facilities for this work, Dr. H. Quader and Professor Z. Hejnowicz for critically reading the manuscript, and H.-J. Ensikat for technical assistance. Supported by the Deutsche Forschungsgemeinschaft.
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Kutschera, U. Cell-wall synthesis and elongation growth in hypocotyls of Helianthus annuus L.. Planta 181, 316–323 (1990). https://doi.org/10.1007/BF00195882
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DOI: https://doi.org/10.1007/BF00195882