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A comparative study into the chemical constitution of cutins and suberins from Picea abies (L.) Karst., Quercus robur L., and Fagus sylvatica L.

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Abstract

The compositions of BF3/CH3OH depolymerisates of cutins and suberins from leaf and periderm samples from Picea abies [L.] Karst., Quercus robur L., and Fagus sylvatica L., respectively, were determined by quantitative capillary gas chromatography/mass spectroscopy. Long-chain monobasic, ω-hydroxymonobasic, dihydroxymonobasic, trihydroxymonobasic and epoxyhydroxymonobasic alkanoic acids constituted the major aliphatic monomers of leaf cutins. The total amounts of cutin monomers ranged from 629 mg · m−2 (Fagus) to 1350 mg · m−2 (Quercus). Cutin composition and amounts did not significantly differ between current year and three-year-old needles of Picea. Trans-esterification of periderm samples yielded a much greater variety of aliphatic monomers than obtained from cutins. In addition to the substance classes found with cutins, suberin depolymerisates also contained α, ω-dibasic acids while dihydroxymonobasic acids were lacking. Depolymerisates from periderms taken from different locations on a Picea tree did not differ significantly in their relative composition. The results are discussed in terms of the distinctive characteristics of the aliphatic portions of cutins and suberins, respectively. Discriminant analysis is applied for formulating a quantitative and inarbitrary classification rule for cutins and suberins. The precision, statistical significance and robustness of this classification rule are tested by employing it to a large set of compositional data (70 plant species) from the literature. The relevance of data obtained by depolymerization methods for elucidating the physical structure of cutins and suberins in situ is evaluated.

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Authors and Affiliations

  1. Institut für Botanik und Mikrobiologie, Technische Universität München, Arcisstrasse 21, 2, München, Germany

    K. Matzke & M. Riederer

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  1. K. Matzke
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  2. M. Riederer
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The authors are indebted to Drs. J. Winkler and H. Krause (Laboratorium für Strukturchemie des Fachbereichs Chemie, Biologie und Geowissenschaften, Technische Universität München, Garching, FRG) for performing capillary gas chromatography-mass spectrometry and their valuable help in the identification of cutin and suberin constituents. The work was supported by grants from the Deutsche Forschungsgemeinschaft and the Bayerisches Staatsministerium für Unterricht, Kultus, Wissenschaft und Kunst.

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Matzke, K., Riederer, M. A comparative study into the chemical constitution of cutins and suberins from Picea abies (L.) Karst., Quercus robur L., and Fagus sylvatica L.. Planta 185, 233–245 (1991). https://doi.org/10.1007/BF00194066

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