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Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture

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Biotechnology Techniques

Summary

The effectiveness of silkworm hemolymph was investigated as a substitute for fetal bovine serum (FBS) in insect cell culture. Cells were adapted to grow in reduced FBS medium supplemented with silkworm hemolymph through a gradual adaptation process. FBS concentration in the medium could be reduced to 1% without decrease in cell growth rate and maximum cell concentration by adding 5% silkworm hemolymph.

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Author notes

  1. Tai Hyun Park

    Present address: Department of Chemical & Biochemical Engineering, University of California, 92717, Irvine, CA, USA

Authors and Affiliations

  1. Department of Genetic Engineering, Sung Kyun Kwan University, 300 Chunchun-dong, 440-746, Suwon, Korea

    Sung Ho Ha & Tai Hyun Park

  2. Division of Genetics, Sericulture & Insect Research Institute, 61 Seodun-dong, 441-100, Suwon, Korea

    Sam-Eun Kim

Authors
  1. Sung Ho Ha
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  2. Tai Hyun Park
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  3. Sam-Eun Kim
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Ha, S.H., Park, T.H. & Kim, SE. Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture. Biotechnol Tech 10, 401–406 (1996). https://doi.org/10.1007/BF00174223

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  • DOI: https://doi.org/10.1007/BF00174223

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