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A method for removal of toxic chromium using dialysis-sac cultures of a chromate-reducing strain of Enterobacter cloacae

  • Environmental Microbiology
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Summary

A method for removal of toxic hexavalent chromium (chlomate: CrO sup2−inf4 ) was developed by use of dialysis-sac cultures of a chromate-reducing strain of Enterobacter cloacae (HO1). E. cloacae strain HO1 cells were put in dialysis (semipermeable membrane) sacs, and the sacs were submerged in water containing toxic CrO sup2−inf4 . The dialysis sacs allowed CrO sup2−inf4 to diffuse into the culture, and CrO sup2−inf4 was reduced anaerobically in the dialysis sacs by the E. cloacae cells. Because reduced chromium readily formed insoluble chromium hydroxides in the dialysis sacs, the greater part of reduced chromium was unable to diffuse out through the semipermeable membrane. Thus the dialysis culture of E. cloacae strain HO1 could successfully remove toxic chromium from the surrounding water. If the initial concentration of CrO sup2−inf4 was less than 4mM (208 ppm as chromium), about 90% of the total chromium could be removed from water by the described method.

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Komori, K., Rivas, A., Toda, K. et al. A method for removal of toxic chromium using dialysis-sac cultures of a chromate-reducing strain of Enterobacter cloacae . Appl Microbiol Biotechnol 33, 117–119 (1990). https://doi.org/10.1007/BF00170582

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  • DOI: https://doi.org/10.1007/BF00170582

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