Bioactive materials such as hydroxyapatite ceramics are known to show a stable interfacial bond with tissues. The objective of this study was to investigate the behaviour of human bone differentiated cells isolated from bone marrow on the surface of a hydroxyapatite ceramic obtained from bovine spongiosa. Scanning electron microscopy and DNA synthesis analysis assessed by [3H]-thymidine incorporation showed cell colonization of the whole material. Immunological studies using monoclonal antibodies to osteocalcin and osteonectin and cytochemical analysis of alkaline phosphatase activity indicated that these cells did not lose their osteoblastic phenotype after 28 days of culture. Furthermore, this study demonstrates the in vitro interface between the material and human cells, which is reporduced in vivo.
Similar content being viewed by others
References
C. BAQUEY and B. DUPUY, “Colloques INSERM” (1989) VII. Foreword.
M. F. HARMAND, 3rd World Biomaterial Congress, Kyoto, Japan, 1988, p. 487.
H. OHGUSHI, Y. DOHY, S. TAMI and S. TABATA, J. Biomed. Mater. Sci. 27 (1993) 1401.
L. L. HENCH, Ann. NY Acad. Sci. 523 (1988) 54.
S. D. TROSTER, in “Jahrbuch der Orthopädie”, edited by Von VENBROCKS G., Von SALIS-SOGLIO (Bierman Verlag, Zülpig, 1993), p. 231.
M. DARD, J. BAUER, A. LIEBENDORFER, H. WAHLIG and E. DINGELDEIN, Acta Odonto. Stom. 185 (1994) 61.
H. S. CHEUNG and M. H. HAAK, Biomaterials 10 (1989) 63.
A. LIFBENDORFER, M. DARD and H. WAHLIG, 3rd Congress of the European Orthopaedic Research Society, Paris 1993, p. 58.
A. LIEBENDORFER, M. DARD and H. WAHLIG, XIX Annual. Meeting of the Society for Biomaterials, Birmingham, USA, 1993, pp 246.
J. VILAMITJANA-AMEDEE, R. BAREILLE, F. ROUAIS, A. I. CAPLAN and M. F. HARMAND, In Vitro Cell. Dev. Biol. 29 (1993) 699.
J. AMEDEE, R. BAREILLE, R. JEANDOT, L. BORDENAVE, M. Remy, F. Rouais and Ch. Baquey, Biomaterials (in press).
P. V. HAUSCHKA, A. E. MACRAKOS, M. D. IAFRATI, S. E. DOLEMAN and M. KLAGSBRUN, J. Biol. Chem. 261 (1986) 12665.
R. MAJESKA and G. RODAN, Calcif. Tissue Int. 34 (1982) 59.
A. WILKE, R. Th NAGEL, M. AHMED, H. KIENAPPEL, J. ORTH, P. GRISS, R. P. FRANKE. 11th European Conference of Biomaterials, Pisa, 10–14 September 1994.
R. SCHNETTLER, E. DINGELDEIN, H. WAHLIG and W. TAUSCH, 4th World Biomaterials Congress Berlin, 1992, p. 260.
G. GUILLEMIN, M. LAUNAY and A. MEUNIER, J. Mater. Sci. 4 (1993) 575.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Faucheux, C., Bareille, R., Rouais, F. et al. Biocompatibility testing of a bovine hydroxy—apatite ceramic material with the use of osteo-progenitor cells isolated from human bone marrow. J Mater Sci: Mater Med 5, 635–639 (1994). https://doi.org/10.1007/BF00120345
Issue Date:
DOI: https://doi.org/10.1007/BF00120345